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Literature summary extracted from

  • Lee, S.A.; Belyaeva, O.V.; Kedishvili, N.Y.
    Biochemical characterization of human epidermal retinol dehydrogenase 2 (2009), Chem. Biol. Interact., 178, 182-187.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.105 expressed in Sf9 insect cells and in HEK-293 cells Homo sapiens
1.1.1.105 RDH-E2 is expressed in Sf9 insect cells as a fusion to the C-terminal His6-tag Homo sapiens

General Stability

EC Number General Stability Organism
1.1.1.105 gel-filtration of RDH-E2 preparation to remove imidazole results in significant loss of activity (about 90%) despite nearly complete recovery of the protein suggesting that RDH-E2 is sensitive to desalting Homo sapiens
1.1.1.105 the enzyme is sensitive to desalting Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.105 imidazole increasing the amount of imidazole in the reaction mixture with purified RDH-E2 from 25 to 225mM significantly decreases RDH-E2 activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.105 endoplasmic reticulum
-
 Homo sapiens 5783
-
1.1.1.105 endoplasmic reticulum RDH-E2 protein exhibits a punctuate localization pattern, surrounding the nucleus suggesting that RDH-E2 is associated with endoplasmic reticulum Homo sapiens 5783
-
1.1.1.105 membrane of endoplasmic reticulum Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.105 all-trans-retinol + NAD+ Homo sapiens exhibits a strong preference for NAD+/NADH as cofactors. Activity toward all-trans-retinal in the presence of NADH is 2fold lower than activity with all-trans-retinol and NAD+. The preference for NAD+ suggests that RDH-E2 is likely to function in the oxidative direction in vivo, further supporting its potential role in the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in human keratinocytes all-trans-retinal + NADH + H+
-
 r
1.1.1.105 additional information Homo sapiens RDH-E2 may be involved in the pathogenesis of psoriasis through its potential role in retinoic acid biosynthesis and stimulation of keratinocyte proliferation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.105 Homo sapiens Q8N3Y7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.105 Ni2+-NTA resin column chromatography Homo sapiens
1.1.1.105 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.105 epidermis retinal dehydrogenase 2 is significantly elevated in psoriatic skin Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.105 all-trans-retinal + NADH + H+
-
 Homo sapiens all-trans-retinol + NAD+
-
 ?
1.1.1.105 all-trans-retinaldehyde + NADH + H+
-
 Homo sapiens ?
-
 ?
1.1.1.105 all-trans-retinol + NAD+ exhibits a strong preference for NAD+/NADH as cofactors. Activity toward all-trans-retinal in the presence of NADH is 2fold lower than activity with all-trans-retinol and NAD+. The preference for NAD+ suggests that RDH-E2 is likely to function in the oxidative direction in vivo, further supporting its potential role in the oxidation of retinol to retinaldehyde for retinoic acid biosynthesis in human keratinocytes Homo sapiens all-trans-retinal + NADH + H+
-
 r
1.1.1.105 all-trans-retinol + NAD+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+. Activity toward all-trans-retinal in the presence of NADH is 2fold lower than activity with all-trans-retinol and NAD+ Homo sapiens all-trans-retinal + NADH + H+
-
 r
1.1.1.105 all-trans-retinol + NADP+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+ Homo sapiens all-trans-retinal + NADPH + H+
-
 r
1.1.1.105 additional information no activity was detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH Homo sapiens ?
-
 ?
1.1.1.105 additional information RDH-E2 may be involved in the pathogenesis of psoriasis through its potential role in retinoic acid biosynthesis and stimulation of keratinocyte proliferation Homo sapiens ?
-
 ?
1.1.1.105 additional information no activity is detected with 11-cis-retinol or 11-cis-retinaldehyde as substrates with either NAD+/NADH or NADP+/NADPH Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.105 epidermal retinol dehydrogenase 2
-
 Homo sapiens
1.1.1.105 RDH-E2
-
 Homo sapiens
1.1.1.105 retinol dehydrogenase 2
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.105 NAD+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+ Homo sapiens
1.1.1.105 NADH exhibits a strong preference for NAD+/NADH as cofactors Homo sapiens
1.1.1.105 NADP+ exhibits a strong preference for NAD+/NADH as cofactors. Activity with NAD+ is about 10fold higher than activity in presence of NADP+ Homo sapiens