EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.8 | into the vectorS pET28a, pEZS-NL and pPCVE35E | Zea mays |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.8 | 0.458 | - |
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.2.8 | plastid | - |
Zea mays | 9536 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.8 | (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | Zea mays | - |
indole + D-glyceraldehyde 3-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.8 | Zea mays | B2Y0K4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.8 | recombinant protein by His-tag affinity purification via Ni-NTA agarose, plant protein by anion exchange and gel permeation chromatography | Zea mays |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.1.2.8 | leaf | - |
Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.8 | (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays | indole + D-glyceraldehyde 3-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.8 | tryptophan synthase alpha subunit | - |
Zea mays |
4.1.2.8 | ZmTSA | - |
Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.8 | 30 | - |
tryptophan synthase assay | Zea mays |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.8 | 0.006 | - |
(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate | - |
Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.8 | 8.2 | - |
tryptophan synthase assay | Zea mays |