Literature summary extracted from

Pires, A.S.; Cabral, M.G.; Fevereiro, P.; Stoger, E.; Abranches, R.
High levels of stable phytase accumulate in the culture medium of transgenic Medicago truncatula cell suspension cultures (2008), Biotechnol. J., 3, 916-923.

Application

EC Number	Application	Comment	Organism
3.1.3.8	synthesis	expression of phytase in a Medicago truncatula cell suspension line. Recombinant phytase accumulates to at least 25 mg/l and remaines stable along the growth curve, and an enriched fraction with high enzymatic activity is easily obtained	Aspergillus niger
3.1.3.26	biotechnology	high levels of stable phytase is expressed in the culture medium of transgenic Medicago truncatula cell suspension cultures	Aspergillus niger

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.3.8	expression in Medicago truncatula	Aspergillus niger
3.1.3.26	enzyme is expressed in a Medicago truncatula cell suspension line. Enzymatically active recombinant phytase is produced and secreted into the culture medium. Recombinant phytase accumulate to at least 25 mg/l and remains stable along the growth curve, and an enriched fraction with high enzymatic activity is easily obtained	Aspergillus niger

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.3.8	70000	-	x * 70000, SDS-PAGE	Aspergillus niger

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.3.8	Aspergillus niger	-	-	-
3.1.3.26	Aspergillus niger	-	the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.3.8	870	-	pH 6.5, 37°C	Aspergillus niger

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.3.8	myo-inositol hexakisphosphate + H2O	-	Aspergillus niger	1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.3.8	?	x * 70000, SDS-PAGE	Aspergillus niger

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.3.26	phytase	-	Aspergillus niger

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Release 2023.1 (January 2023)