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Literature summary extracted from
- Identification and characterization of the bacterial D-gluconate dehydratase in Achromobacter xylosoxidans (2008), Biotechnol. Bioprocess Eng., 13, 436-444.
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.39 | into the pGEM-T easy vector for sequencing | Achromobacter xylosoxidans |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.39 | 62000 | - |
determined by SDS-PAGE | Achromobacter xylosoxidans |
| 4.2.1.39 | 63410 | - |
calculated | Achromobacter xylosoxidans |
| 4.2.1.39 | 270000 | - |
native enzyme, tetramer | Achromobacter xylosoxidans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.39 | D-gluconate | Achromobacter xylosoxidans | substrate specificity 100% | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.39 | Achromobacter xylosoxidans | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.39 | after ammonium sulfate precipitation chromatography on a Phenyl-Sepharose, a Q-Sepharose, a Mono Q, and a HiTrap desalting column is performed | Achromobacter xylosoxidans |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.39 | 0.002 | - |
nitrogen source urea | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.011 | - |
carbon source DL-glutamate | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.013 | - |
carbon source DL-alanine | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.019 | - |
nitrogen source bacto peptone | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.025 | - |
carbon source D-glucose | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.027 | - |
carbon source D-galactose | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.027 | - |
nitrogen source yeast extract | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.031 | - |
nitrogen source (NH4)SO4 | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.031 | - |
nitrogen source glucosamine | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.036 | - |
nitrogen source H4NO3 | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.038 | - |
carbon source D-galactonate | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.04 | - |
nitrogen source casamino acid | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.042 | - |
carbon source maltose | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.046 | - |
carbon source D-arabinose | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.05 | - |
nitrogen source NH4Cl | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.057 | - |
carbon source sucrose | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.111 | - |
nitrogen source malt extract | Achromobacter xylosoxidans |
| 4.2.1.39 | 0.13 | - |
carbon source D-gluconate | Achromobacter xylosoxidans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.39 | D-arabonate | substrate specificity 4% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | D-fuconate | substrate specificity 18% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | D-galactonate | substrate specificity 14% | Achromobacter xylosoxidans | 2-dehydro-3-deoxy-D-galactonate + H2O | - |
? | |
| 4.2.1.39 | D-gluconate | substrate specificity 100% | Achromobacter xylosoxidans | 2-dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
| 4.2.1.39 | D-mannonate | substrate specificity 13% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | D-ribonate | substrate specificity 11% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | D-xylonate | substrate specificity 82% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | DL-isovalerate | substrate specificity 11% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | L-arabonate | substrate specificity 2% | Achromobacter xylosoxidans | ? | - |
? | |
| 4.2.1.39 | L-mannonate | substrate specificity 10% | Achromobacter xylosoxidans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.39 | homotetramer | - |
Achromobacter xylosoxidans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.39 | D-gluconate dehydratase | - |
Achromobacter xylosoxidans |
| 4.2.1.39 | gluconate dehydratase | - |
Achromobacter xylosoxidans |
| 4.2.1.39 | GNAD | - |
Achromobacter xylosoxidans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.39 | 30 | - |
standard assay condition | Achromobacter xylosoxidans |
| 4.2.1.39 | 50 | - |
optimal activity | Achromobacter xylosoxidans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.39 | 7.5 | 9 | - |
Achromobacter xylosoxidans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.39 | 7.5 | - |
standard assay condition | Achromobacter xylosoxidans |
| 4.2.1.39 | 8.5 | - |
optimal activity | Achromobacter xylosoxidans |
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Release 2023.1 (January 2023)
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