BRENDA - Enzyme Database

Identification and characterization of the bacterial D-gluconate dehydratase in Achromobacter xylosoxidans

Kim, S.; Lee, S.B.; Biotechnol. Bioprocess Eng. 13, 436-444 (2008)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.39
into the pGEM-T easy vector for sequencing
Achromobacter xylosoxidans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.39
62000
-
determined by SDS-PAGE
Achromobacter xylosoxidans
4.2.1.39
63410
-
calculated
Achromobacter xylosoxidans
4.2.1.39
270000
-
native enzyme, tetramer
Achromobacter xylosoxidans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.39
D-gluconate
Achromobacter xylosoxidans
substrate specifity 100%
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.39
Achromobacter xylosoxidans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.39
after ammonium sulfate precipitation chromatography on a Phenyl-Sepharose, a Q-Sepharose, a Mono Q, and a HiTrap desalting column is performed
Achromobacter xylosoxidans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.39
0.002
-
nitrogen source urea
Achromobacter xylosoxidans
4.2.1.39
0.011
-
carbon source DL-glutamate
Achromobacter xylosoxidans
4.2.1.39
0.013
-
carbon source DL-alanine
Achromobacter xylosoxidans
4.2.1.39
0.019
-
nitrogen source bacto peptone
Achromobacter xylosoxidans
4.2.1.39
0.025
-
carbon source D-glucose
Achromobacter xylosoxidans
4.2.1.39
0.027
-
carbon source D-galactose; nitrogen source yeast extract
Achromobacter xylosoxidans
4.2.1.39
0.031
-
nitrogen source glucosamine; nitrogen source (NH4)SO4
Achromobacter xylosoxidans
4.2.1.39
0.036
-
nitrogen source H4NO3
Achromobacter xylosoxidans
4.2.1.39
0.038
-
carbon source D-galactonate
Achromobacter xylosoxidans
4.2.1.39
0.04
-
nitrogen source casamino acid
Achromobacter xylosoxidans
4.2.1.39
0.042
-
carbon source maltose
Achromobacter xylosoxidans
4.2.1.39
0.046
-
carbon source D-arabinose
Achromobacter xylosoxidans
4.2.1.39
0.05
-
nitrogen source NH4Cl
Achromobacter xylosoxidans
4.2.1.39
0.057
-
carbon source sucrose
Achromobacter xylosoxidans
4.2.1.39
0.111
-
nitrogen source malt extract
Achromobacter xylosoxidans
4.2.1.39
0.13
-
carbon source D-gluconate
Achromobacter xylosoxidans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.39
D-arabonate
substrate specifity 4%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-fuconate
substrate specifity 18%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-galactonate
substrate specifity 14%
691429
Achromobacter xylosoxidans
2-dehydro-3-deoxy-D-galactonate + H2O
-
-
-
?
4.2.1.39
D-gluconate
substrate specifity 100%
691429
Achromobacter xylosoxidans
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
-
?
4.2.1.39
D-mannonate
substrate specifity 13%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-ribonate
substrate specifity 11%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-xylonate
substrate specifity 82%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
DL-isovalerate
substrate specifity 11%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
L-arabonate
substrate specifity 2%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
L-mannonate
substrate specifity 10%
691429
Achromobacter xylosoxidans
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.39
homotetramer
-
Achromobacter xylosoxidans
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.39
30
-
standard assay condition
Achromobacter xylosoxidans
4.2.1.39
50
-
optimal activity
Achromobacter xylosoxidans
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.39
7.5
9
-
Achromobacter xylosoxidans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.39
7.5
-
standard assay condition
Achromobacter xylosoxidans
4.2.1.39
8.5
-
optimal activity
Achromobacter xylosoxidans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.39
into the pGEM-T easy vector for sequencing
Achromobacter xylosoxidans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.39
62000
-
determined by SDS-PAGE
Achromobacter xylosoxidans
4.2.1.39
63410
-
calculated
Achromobacter xylosoxidans
4.2.1.39
270000
-
native enzyme, tetramer
Achromobacter xylosoxidans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.39
D-gluconate
Achromobacter xylosoxidans
substrate specifity 100%
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.39
after ammonium sulfate precipitation chromatography on a Phenyl-Sepharose, a Q-Sepharose, a Mono Q, and a HiTrap desalting column is performed
Achromobacter xylosoxidans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.39
0.002
-
nitrogen source urea
Achromobacter xylosoxidans
4.2.1.39
0.011
-
carbon source DL-glutamate
Achromobacter xylosoxidans
4.2.1.39
0.013
-
carbon source DL-alanine
Achromobacter xylosoxidans
4.2.1.39
0.019
-
nitrogen source bacto peptone
Achromobacter xylosoxidans
4.2.1.39
0.025
-
carbon source D-glucose
Achromobacter xylosoxidans
4.2.1.39
0.027
-
carbon source D-galactose; nitrogen source yeast extract
Achromobacter xylosoxidans
4.2.1.39
0.031
-
nitrogen source glucosamine; nitrogen source (NH4)SO4
Achromobacter xylosoxidans
4.2.1.39
0.036
-
nitrogen source H4NO3
Achromobacter xylosoxidans
4.2.1.39
0.038
-
carbon source D-galactonate
Achromobacter xylosoxidans
4.2.1.39
0.04
-
nitrogen source casamino acid
Achromobacter xylosoxidans
4.2.1.39
0.042
-
carbon source maltose
Achromobacter xylosoxidans
4.2.1.39
0.046
-
carbon source D-arabinose
Achromobacter xylosoxidans
4.2.1.39
0.05
-
nitrogen source NH4Cl
Achromobacter xylosoxidans
4.2.1.39
0.057
-
carbon source sucrose
Achromobacter xylosoxidans
4.2.1.39
0.111
-
nitrogen source malt extract
Achromobacter xylosoxidans
4.2.1.39
0.13
-
carbon source D-gluconate
Achromobacter xylosoxidans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.39
D-arabonate
substrate specifity 4%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-fuconate
substrate specifity 18%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-galactonate
substrate specifity 14%
691429
Achromobacter xylosoxidans
2-dehydro-3-deoxy-D-galactonate + H2O
-
-
-
?
4.2.1.39
D-gluconate
substrate specifity 100%
691429
Achromobacter xylosoxidans
2-dehydro-3-deoxy-D-gluconate + H2O
-
-
-
?
4.2.1.39
D-mannonate
substrate specifity 13%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-ribonate
substrate specifity 11%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
D-xylonate
substrate specifity 82%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
DL-isovalerate
substrate specifity 11%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
L-arabonate
substrate specifity 2%
691429
Achromobacter xylosoxidans
?
-
-
-
?
4.2.1.39
L-mannonate
substrate specifity 10%
691429
Achromobacter xylosoxidans
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.39
homotetramer
-
Achromobacter xylosoxidans
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.39
30
-
standard assay condition
Achromobacter xylosoxidans
4.2.1.39
50
-
optimal activity
Achromobacter xylosoxidans
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.39
7.5
9
-
Achromobacter xylosoxidans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.39
7.5
-
standard assay condition
Achromobacter xylosoxidans
4.2.1.39
8.5
-
optimal activity
Achromobacter xylosoxidans