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Literature summary extracted from

  • Goerke, A.R.; Swartz, J.R.
    High-level cell-free synthesis yields of proteins containing site-specific non-natural amino acids (2008), Biotechnol. Bioeng., 102, 400-416.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.1.1.1 biotechnology Escherichia coli-based cell-free system for the production of proteins with a non-natural amino acid incorporated site-specifically is described. A mutant Methanococcus jannaschii tyrosyl-tRNA synthetase (mTyrRS) and tRNATyr pair are used as orthogonal elements. The mTyrRS experienced proteolysis and modified protein yields improves with higher synthetase addition (200-300 mg/mL) Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.1 expressed in Escherichia coli Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Methanocaldococcus jannaschii
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Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 Tyrosyl-tRNA synthetase
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Methanocaldococcus jannaschii