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Literature summary extracted from

  • Zavrel, M.; Schmidt, T.; Michalik, C.; Ansorge-Schumacher, M.; Marquardt, W.; Buechs, J.; Spiess, A.C.
    Mechanistic kinetic model for symmetric carboligations using benzaldehyde lyase (2008), Biotechnol. Bioeng., 101, 27-38.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 for expression in Escherichia coli cells Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.38 0.005
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for second binding substrate, mechanistic model Pseudomonas fluorescens
4.1.2.38 0.007
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for second binding substrate, mechanistic model Pseudomonas fluorescens
4.1.2.38 0.01
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for first binding substrate, simplified model Pseudomonas fluorescens
4.1.2.38 0.012
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for first binding substrate, simplified model Pseudomonas fluorescens
4.1.2.38 0.042
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for second binding substrate, simplified model Pseudomonas fluorescens
4.1.2.38 0.047
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for second binding substrate, simplified model Pseudomonas fluorescens
4.1.2.38 0.05
-
 3,5-dimethoxy-benzaldehyde Michaelis constant for first binding substrate, mechanistic model Pseudomonas fluorescens
4.1.2.38 0.975
-
 (R)-3,3',5,5'-tetramethoxy-benzoin Michaelis constant for product, mechanistic model Pseudomonas fluorescens
4.1.2.38 1.205
-
 (R)-3,3',5,5'-tetramethoxy-benzoin Michaelis constant for product, mechanistic model Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+
-
 Pseudomonas fluorescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.38 58919
-
 4 * 58919 Pseudomonas fluorescens
4.1.2.38 58920
-
 monomer, calculated from sequence Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.38 2-hydroxy-1,2-diphenylethanone Pseudomonas fluorescens
-
 benzaldehyde
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.38 using Ni2+-affinity chromatography Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 2-hydroxy-1,2-diphenylethanone
-
 Pseudomonas fluorescens benzaldehyde
-
 r
4.1.2.38 3,5-dimethoxy-benzaldehyde
-
 Pseudomonas fluorescens (R)-3,3',5,5'-tetramethoxy-benzoin
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.38 homotetramer 4 * 58919 Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens
4.1.2.38 benzaldehyde lyase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.38 25
-
 activity assay Pseudomonas fluorescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.38 45.44
-
 3,5-dimethoxy-benzaldehyde simplified model Pseudomonas fluorescens
4.1.2.38 46.12
-
 3,5-dimethoxy-benzaldehyde mechanistic model Pseudomonas fluorescens
4.1.2.38 46.15
-
 3,5-dimethoxy-benzaldehyde mechanistic model Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 8.5
-
 activity assay Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate
-
 Pseudomonas fluorescens