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Literature summary extracted from

  • Barshan-Tashnizi, M.; Ahmadian, S.; Niknam, K.; Torabi, S.F.; Ranaei-Siadat, S.O.
    Covalent immobilization of Drosophila acetylcholinesterase for biosensor applications (2008), Biotechnol. Appl. Biochem., 52, 257-264.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 AChE expression in Spodoptera frugiperda Sf9 cells using the baculovirus transfection method Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 additional information immobilization of AChE on preactivated perlite, a porous silica matrix, by silanization and glutaraldehyde treatment, the immobilization leads to 30% reduced specific activity and improved enzyme stability, e.g. against urea, acetonitrile and temperature, immobilized enzymes retain 80% of its initial activity after 16 consecutive reactor batch cycles, overview Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 paraoxon
-
 Drosophila melanogaster
3.1.1.7 Urea the stability of the immobilized recombinant enzyme is 2.7fold increased compared to the soluble recombinant enzyme Drosophila melanogaster

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.7 acetonitril the stability of the immobilized recombinant enzyme is 1.7fold increased compared to the soluble recombinant enzyme Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 recombinant AChE from Spodoptera frugiperda Sf9 cells Drosophila melanogaster

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 26240
-
 purified recombinant immobilized enzyme Drosophila melanogaster
3.1.1.7 36370
-
 purified recombinant enzyme Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.7 S-acetylthiocholine + H2O detection with 5,5'-dithio-bis-2-nitrobenzoic acid, i.e. DTNB Drosophila melanogaster acetate + thiocholine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
 Drosophila melanogaster

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.7 50
-
 the stability of the immobilized recombinant enzyme is 8.7fold increased compared to the soluble recombinant enzyme Drosophila melanogaster
3.1.1.7 60
-
 the stability of the immobilized recombinant enzyme is 17.7fold increased compared to the soluble recombinant enzyme Drosophila melanogaster

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.4
-
 assay at Drosophila melanogaster