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Literature summary extracted from

  • Synstad, B.; Vaaje-Kolstad, G.; Cederkvist, F.H.; Saua, S.F.; Horn, S.J.; Eijsink, V.G.; Sorlie, M.
    Expression and characterization of endochitinase C from Serratia marcescens BJL200 and its purification by a one-step general chitinase purification method (2008), Biosci. Biotechnol. Biochem., 72, 715-723.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chiC, expression of ChiC in Escherichia coli strain BL21(DE3) periplasm Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information pH dependence of ChiC kinetics, overview Serratia marcescens
3.2.1.14 0.0042
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside pH 6.5, isozyme ChiA Serratia marcescens
3.2.1.14 0.0068
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 6.5, isozyme ChiB Serratia marcescens
3.2.1.14 0.0798
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 6.5, isozyme ChiC Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Serratia marcescens Q700B8 genes chiA, chiB, and chiC encoding isozymes BJL200-ChiA, BJL200-ChiB, and BJL200-ChiC, respectively
-
3.2.1.14 Serratia marcescens BJL200 Q700B8 genes chiA, chiB, and chiC encoding isozymes BJL200-ChiA, BJL200-ChiB, and BJL200-ChiC, respectively
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant isozyme ChiC from Escherichia coli strain BL21(DE3) periplasm in a one step procedure using hydrophobic interaction chromatography Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
-
 Serratia marcescens
3.2.1.14 0.0123
-
 purified recombinant ChiC Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate Serratia marcescens ?
-
 ?
3.2.1.14 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside + H2O BJL200-ChiA and BJL200-ChiB have an approximately 30fold higher kcat and 15fold lower Km than BJL200-ChiC1 for the oligomeric substrate Serratia marcescens BJL200 ?
-
 ?
3.2.1.14 CM-chitin-RBV + H2O BJL200-ChiC1 is 10-15times faster than BJL200-ChiB and BJL200-ChiA in degrading the polymeric substrate CM-chitin-RBV Serratia marcescens ?
-
 ?
3.2.1.14 CM-chitin-RBV + H2O BJL200-ChiC1 is 10-15times faster than BJL200-ChiB and BJL200-ChiA in degrading the polymeric substrate CM-chitin-RBV Serratia marcescens BJL200 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 BJL200-ChiC1
-
 Serratia marcescens
3.2.1.14 chitinase C1
-
 Serratia marcescens
3.2.1.14 More BJL200-ChiC1 belongs to the family 18 chitinases Serratia marcescens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 2
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 6.5, isozyme ChiC Serratia marcescens
3.2.1.14 56.8
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 6.5, isozyme ChiB Serratia marcescens
3.2.1.14 67.4
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 6.5, isozyme ChiA Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 3.9
-
 with substrate 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside, isozyme ChiC Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 additional information
-
 pH dependence of ChiC kinetics, overview Serratia marcescens