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Literature summary extracted from

  • Shirota, K.; Sato, T.; Sekiguchi, J.; Miyauchi, K.; Mochizuki, A.; Matsumiya, M.
    Purification and characterization of chitinase isozymes from a red algae, Chondrus verrucosus (2008), Biosci. Biotechnol. Biochem., 72, 3091-3099.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 24500
-
 x * 24500, isozymes ChiA and ChiC, SDS-PAGE, x * 25500, isozyme ChiB, SDS-PAGE Chondrus verrucosus
3.2.1.14 25500
-
 x * 24500, isozymes ChiA and ChiC, SDS-PAGE, x * 25500, isozyme ChiB, SDS-PAGE Chondrus verrucosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Chondrus verrucosus
-
 isozymes ChiA, ChiB, and ChiC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme by ammonium sulfate fractionation, dialysis, anion exchange and hydroxyapatite chromatography, followed by another step of anion exchange chromatography Chondrus verrucosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 CM-chitin-RBV + H2O polymeric substrate CM-chitin-RBV Chondrus verrucosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAc + H2O
-
 Chondrus verrucosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Chondrus verrucosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Chondrus verrucosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O substrate of isozymes ChiA, ChiB, and ChiC with formation of (N-acetylglucosamine)n and n = 2 to 4 Chondrus verrucosus ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O substrate of isozymes ChiA, ChiB, and ChiC with formation of (N-acetylglucosamine)n and n = 2 to 4 Chondrus verrucosus ?
-
 ?
3.2.1.14 additional information isozymes ChiA, ChiB, and ChiC degrade squid pen beta-chitin to a higher degree than shrimp shell or crab shell alpha-chitin, isozyme specificity towards soluble substrates, overview. No activity with 4-nitrophenyl-(GlcNAc)3. General sctivity of isozymes in descending order: ChiA, ChiC, ChiB Chondrus verrucosus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 24500, isozymes ChiA and ChiC, SDS-PAGE, x * 25500, isozyme ChiB, SDS-PAGE Chondrus verrucosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 70
-
 isozyme ChiB Chondrus verrucosus
3.2.1.14 80
-
 isozymes ChiA and ChiC Chondrus verrucosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 additional information
-
 isozyme temperature profiles, overview Chondrus verrucosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 1
-
 isozyme ChiB Chondrus verrucosus
3.2.1.14 2
-
 isozymes ChiA and ChiC Chondrus verrucosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 additional information
-
 isozyme pH profiles, overview Chondrus verrucosus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Chondrus verrucosus isozymes ChiA and ChiC
-
 3.5
3.2.1.14 Chondrus verrucosus isozyme ChiB
-
 4.6