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Literature summary extracted from

  • Ueatrongchit, T.; Kayo, A.; Komeda, H.; Asano, Y.; H-Kittikun, A.
    Purification and characterization of a novel (R)-hydroxynitrile lyase from Eriobotrya japonica (Loquat) (2008), Biosci. Biotechnol. Biochem., 72, 1513-1522.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.10 2-mercaptoethanol 10 mM, 19% inhibition Rhaphiolepis bibas
4.1.2.10 AgNO3 1 mM, 100% inhibition Rhaphiolepis bibas
4.1.2.10 CuSO4 1 mM, 100% inhibition Rhaphiolepis bibas
4.1.2.10 diethyldicarbonate 1 mM, 36% inhibition Rhaphiolepis bibas
4.1.2.10 FeCl3 1 mM, 50% inhibition Rhaphiolepis bibas
4.1.2.10 HgCl2 1 mM, 100% inhibition Rhaphiolepis bibas
4.1.2.10 iodoacetic acid 1 mM, 53% inhibition Rhaphiolepis bibas
4.1.2.10 Phenylmethylsulfonylfluoride 1 mM, 59% inhibition Rhaphiolepis bibas

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.10 0.161
-
 (R)-mandelonitrile pH 5.5 Rhaphiolepis bibas
4.1.2.10 1.1
-
 2-naphthaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 2.6
-
 piperonal pH 5.5 Rhaphiolepis bibas
4.1.2.10 3.5
-
 p-anisaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 11.5
-
 benzaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 39.3
-
 pivaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 41.8
-
 Isobutyraldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 99.9
-
 propionaldehyde pH 5.5 Rhaphiolepis bibas

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.10 additional information metal ions are not required for its activity Rhaphiolepis bibas

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.10 62300
-
 1 * 62300, SDS-PAGE Rhaphiolepis bibas
4.1.2.10 72000
-
 gel filtration Rhaphiolepis bibas

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Rhaphiolepis bibas B7VF77
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.1.2.10 glycoprotein
-
 Rhaphiolepis bibas

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10 to homogeneity. The final yield, of 36% with 49fold purification, is obtained by 30-80% (NH4)2SO4 fractionation and column chromatography on DEAE-Toyopearl and concanavalin A Sepharose 4B, which suggests the presence of a carbohydrate side chain Rhaphiolepis bibas

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seed
-
 Rhaphiolepis bibas
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.10 40.9
-
-
 Rhaphiolepis bibas

Storage Stability

EC Number Storage Stability Organism
4.1.2.10 4°C, 20 mM potassium phosphate buffer, pH 6.0, stable for 30 d Rhaphiolepis bibas

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 cyanide + 2-naphthaldehyde
-
 Rhaphiolepis bibas (R)-2-hydroxy-2-(naphthalen-2-yl)acetonitrile
-
 ?
4.1.2.10 cyanide + benzaldehyde
-
 Rhaphiolepis bibas (R)-mandelonitrile
-
 r
4.1.2.10 cyanide + isobutyraldehyde
-
 Rhaphiolepis bibas 2-hydroxy-3-methylbutyronitrile
-
 ?
4.1.2.10 cyanide + p-anisaldehyde
-
 Rhaphiolepis bibas (R)-2-hydroxy-2-(4-methoxyphenyl)acetonitrile
-
 ?
4.1.2.10 cyanide + piperonal
-
 Rhaphiolepis bibas (R)-2-hydroxy-2-(3,4-methylenedioxyphenyl)acetonitrile
-
 ?
4.1.2.10 cyanide + pivaldehyde
-
 Rhaphiolepis bibas pivaloyl cyanide
-
 ?
4.1.2.10 cyanide + propionaldehyde
-
 Rhaphiolepis bibas 2-hydroxybutyronitrile
-
 ?
4.1.2.10 additional information the enzyme is active towards aromatic and aliphatic aldehydes and shows a preference for small substrates over bulky one Rhaphiolepis bibas ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.10 monomer 1 * 62300, SDS-PAGE Rhaphiolepis bibas

Synonyms

EC Number Synonyms Comment Organism
4.1.2.10 (R)-hydroxynitrile lyase
-
 Rhaphiolepis bibas
4.1.2.10 EjHNL
-
 Rhaphiolepis bibas
4.1.2.10 HNL
-
 Rhaphiolepis bibas

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.10 40
-
-
 Rhaphiolepis bibas

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.2.10 20 60 20°C: about 50% of maximal activity, 60°C: about 40% of maximal activity Rhaphiolepis bibas

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.10 70
-
 60 min, 68% residual activity Rhaphiolepis bibas
4.1.2.10 80
-
 60 min, 28% residual activity Rhaphiolepis bibas

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.10 56
-
 (R)-mandelonitrile pH 5.5 Rhaphiolepis bibas
4.1.2.10 110
-
 Isobutyraldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 164
-
 pivaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 223
-
 propionaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 334
-
 piperonal pH 5.5 Rhaphiolepis bibas
4.1.2.10 582
-
 p-anisaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 1160
-
 2-naphthaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 1700
-
 benzaldehyde pH 5.5 Rhaphiolepis bibas

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.10 5.5
-
-
 Rhaphiolepis bibas

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.10 4.5 6 pH 4.5: about 40% of maximal activity, pH 6.0: about 30% of maximal activity Rhaphiolepis bibas

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.10 3 9 60 min, stable Rhaphiolepis bibas

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.10 FAD enzyme is a flavoprotein containing FAD as a prosthetic group Rhaphiolepis bibas

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.10 9.6
-
 Isobutyraldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 14.3
-
 pivaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 19.4
-
 propionaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 29.2
-
 piperonal pH 5.5 Rhaphiolepis bibas
4.1.2.10 50.8
-
 p-anisaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 101
-
 2-naphthaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 148
-
 benzaldehyde pH 5.5 Rhaphiolepis bibas
4.1.2.10 348
-
 (R)-mandelonitrile pH 5.5 Rhaphiolepis bibas