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Literature summary extracted from

  • Masai, E.; Yamamoto, Y.; Inoue, T.; Takamura, K.; Hara, H.; Kasai, D.; Katayama, Y.; Fukuda, M.
    Characterization of ligV essential for catabolism of vanillin by Sphingomonas paucimobilis SYK-6 (2007), Biosci. Biotechnol. Biochem., 71, 2487-2492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.67 cosmid library of SYK-6 strain transferred to Sphingomonas paucimobilis IAM12578. LigV expressed in Escherichia coli BL21(DE3) Sphingomonas paucimobilis

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.67 additional information insertional inactivation of ligV negatively affects growth on vanillin (only 11% of the vanillin dehydrogenase activity of the wild-type strain), whereas growth on syringaldehyde, veratraldehyde and coniferyl aldehyde is almost the same as the wild-type activity. Dehydrogenase activity towards benzaldehyde, p-hydroxybenzaldehyde, protocatechualdehyde and m-anisaldehyde is decreased to 27%, 35%, 14% and 54%, respectively of the wild-type activity. Mutant retains ability to grow on ferulate Sphingomonas paucimobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.67 50301
-
 x * 50301, SDS-PAGE Sphingomonas paucimobilis

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.67 Sphingomonas paucimobilis A2PZP3
-
-
1.2.1.67 Sphingomonas paucimobilis SYK-6 A2PZP3
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.67 benzaldehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis benzoate + NADH + H+
-
 ?
1.2.1.67 benzaldehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis SYK-6 benzoate + NADH + H+
-
 ?
1.2.1.67 coniferyl aldehyde + NAD+ + H2O activity toward coniferyl aldehyde is less than 5% of that toward vanillin Sphingomonas paucimobilis (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+
-
 ?
1.2.1.67 coniferyl aldehyde + NAD+ + H2O activity toward coniferyl aldehyde is less than 5% of that toward vanillin Sphingomonas paucimobilis SYK-6 (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + NADH + H+
-
 ?
1.2.1.67 m-anisaldehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis 3-methoxybenzoate + NADH + H+
-
 ?
1.2.1.67 m-anisaldehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis SYK-6 3-methoxybenzoate + NADH + H+
-
 ?
1.2.1.67 p-hydroxybenzaldehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis 4-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.67 p-hydroxybenzaldehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis SYK-6 4-hydroxybenzoate + NADH + H+
-
 ?
1.2.1.67 protocatechualdehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis protocatechuate + NADH + H+
-
 ?
1.2.1.67 protocatechualdehyde + NAD+ + H2O
-
 Sphingomonas paucimobilis SYK-6 protocatechuate + NADH + H+
-
 ?
1.2.1.67 syringaldehyde + NAD+ + H2O activity toward syringaldehyde is less than 5% of that toward vanillin Sphingomonas paucimobilis syringate + NADH + H+
-
 ?
1.2.1.67 vanillin + NAD+ + H2O best substrate Sphingomonas paucimobilis vanillate + NADH + H+
-
 ?
1.2.1.67 veratraldehyde + NAD+ + H2O activity toward veratraldehyde is less than 5% of that toward vanillin Sphingomonas paucimobilis veratrate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.67 ? x * 50301, SDS-PAGE Sphingomonas paucimobilis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.67 LigV
-
 Sphingomonas paucimobilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.67 NAD+ prefers NAD+ to NADP+ Sphingomonas paucimobilis
1.2.1.67 NADP+
-
 Sphingomonas paucimobilis