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Literature summary extracted from

  • Mhetras, N.C.; Bastawde, K.B.; Gokhale, D.V.
    Purification and characterization of acidic lipase from Aspergillus niger NCIM 1207 (2008), Bioresour. Technol., 100, 1486-1490.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide complete inhibition at 50 mM Aspergillus niger
3.1.1.3 2,4,6-Trinitrobenzenesulfonic acid 55% residual activity at 2 mM Aspergillus niger
3.1.1.3 diethyldicarbonate complete inhibition at 2 mM Aspergillus niger
3.1.1.3 additional information not inhibited by citraconic anhydride (5 mM), N-ethylmaleimide (10 mM), iodoacetate (10 mM), N-acetylimidazole (10 mM), and phenylglyoxal (10 mM) Aspergillus niger
3.1.1.3 N-bromosuccinimide complete inhibition at 0.001 mM Aspergillus niger
3.1.1.3 phenylmethylsulfonyl fluoride 18% residual activity at 5 mM Aspergillus niger

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 32000
-
 SDS-PAGE Aspergillus niger
3.1.1.3 32200
-
 1 * 32200, MALDI-TOF mass spectrometry Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Aspergillus niger
-
-
-
3.1.1.3 Aspergillus niger NCIM 1207
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ammonium sulfate precipitation, phenyl Sepharose column chromatography, and Sephacryl-100 gel filtration Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Aspergillus niger 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Aspergillus niger NCIM 1207 4-nitrophenol + palmitate
-
 ?
3.1.1.3 triolein + H2O
-
 Aspergillus niger 1,2-diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Aspergillus niger NCIM 1207 1,2-diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 monomer 1 * 32200, MALDI-TOF mass spectrometry Aspergillus niger

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 acidic lipase
-
 Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 50
-
-
 Aspergillus niger

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 40 70 the activity decreases sharply above the optimum temperature with almost 95% loss of its original activity at 70°C, the lipase is stable at 40°C for 3 h, incubation at 50°C for 1 h results in 52% loss of lipase activity Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 2.5
-
-
 Aspergillus niger

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Aspergillus niger isoelectric focusing
-
 8.5