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Literature summary extracted from

  • Simkhada, J.R.; Lee, H.J.; Jang, S.Y.; Kim, J.H.; Lee, H.C.; Sohng, J.K.; Yoo, J.C.
    A novel low molecular weight phospholipase D from Streptomyces sp. CS684 (2009), Biores. Technol., 100, 1388-1393.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.4 CHAPS relative activity: 195% Streptomyces sp.
3.1.4.4 deoxycholic acid relative activity: 273% Streptomyces sp.
3.1.4.4 Triton X-100 relative activity: 292% Streptomyces sp.
3.1.4.4 Tween-20 relative activity: 345% Streptomyces sp.
3.1.4.4 Tween-80 relative activity: 292% Streptomyces sp.

Application

EC Number Application Comment Organism
3.1.4.4 biotechnology the feature of the enzyme, to be highly active in moderate pH and temperature, makes PLD684 easy to handle and thus substantiate its potential biocatalytic application in the industrial scale Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.4 polyoxylethylene-4-laurylether relative activity: 3% Streptomyces sp.
3.1.4.4 SDS relative activity: 41% Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.4 1.16
-
 phosphatidylcholine pH 6.0, 45°C Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.4 29000
-
 SDS-PAGE Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4 using ammonium sulfate fractionation, gel filtration with sepharose CL-6B, anion-exchange chromatography with DEAE-sepharose CL-6B and a second gel filtration with sepharose CL-6B Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 phosphatidylcholine + glycerol catalyzes the transphosphatidylation of glycerol, but not that of L-serine, myo-inositol or ethanolamine Streptomyces sp. phosphatidylglycerol + choline
-
 ?
3.1.4.4 phosphatidylcholine + H2O
-
 Streptomyces sp. choline + phosphatidate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.4 ? x * 29000, SDS-PAGE Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D
-
 Streptomyces sp.
3.1.4.4 PLD684
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 45
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.4 45
-
 enzyme ist stable at or below 45°C when assayed after 40 h and 2 h, respectively Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 6
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.4 5 8 over 95% activity detected Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.4.4 7 9 activity is highly stable at 1°C when measured after 40 h Streptomyces sp.