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Literature summary extracted from

  • Fernandez-Lorente, G.; Godoy, C.A.; Mendes, A.A.; Lopez-Gallego, F.; Grazu, V.; de Las Rivas, B.; Palomo, J.M.; Hermoso, J.; Fernandez-Lafuente, R.; Guisan, J.M.
    Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose (2008), Biomacromolecules, 9, 2553-2561.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli Geobacillus thermocatenulatus

General Stability

EC Number General Stability Organism
3.1.1.3 the aminated enzyme immobilized on glyoxyl-agarose by multipoint covalent attachment retains 65% of the starting activity, the optimal stabilization protocol is the immobilization of aminated BTL2 at pH 9 and the further incubation for 24 h at 25°C and pH 10, this preparation is 5fold more stable than the optimal BTL2 immobilized on glyoxyl agarose and around 1200fold more stable than the enzyme immobilized on CNBr and further aminated Geobacillus thermocatenulatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermocatenulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 butyl-Sepharose column chromatography Geobacillus thermocatenulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 (R,S)-2-O-butyryl-2-phenylacetate + H2O Enantioselectivity of the reaction can be obtained with various methods of enzyme immoblisation and reaction conditions Geobacillus thermocatenulatus (R)-2-O-butyryl-2-phenylacetate + (2S)-hydroxy(phenyl)ethanoic acid + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Geobacillus thermocatenulatus 4-nitrophenol + butyrate
-
 ?
3.1.1.3 diethyl 3-phenylglutarate + H2O
-
 Geobacillus thermocatenulatus (S)-ethyl phenylglutarate + (R)-ethyl phenylglutarate + ethanol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 BTL2
-
 Geobacillus thermocatenulatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 65
-
 the optimal preparation retains 100% of its activity after 100 h of incubation at pH 7 and 65°C Geobacillus thermocatenulatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 7
-
 the optimal preparation retains 100% of its activity after 100 h of incubation at pH 7 and 65°C Geobacillus thermocatenulatus