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Literature summary extracted from

  • Collard, F.; Vertommen, D.; Fortpied, J.; Duester, G.; Van Schaftingen, E.
    Identification of 3-deoxyglucosone dehydrogenase as aldehyde dehydrogenase 1A1 (retinaldehyde dehydrogenase 1) (2007), Biochimie, 89, 369-373.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.36 degradation ALDH1A1 appears to be the major if not the only enzyme responsible for the oxidation of 3-deoxyglucosone to 2-keto-3-deoxygluconate Mus musculus
1.2.1.36 degradation urinary excretion of 2-keto-3-deoxygluconate amounts to 16.7 micromol/g creatinine in humans, indicating that 3-deoxyglucosone may be quantitatively a more important substrate than retinaldehyde for ALDH1A1 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.36 ligated into a pCMV5 expression vector and overexpressed in HEK-293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.36 additional information 3-deoxyglucosone dehydrogenase activity is undetectable in tissues from ALDH1A1-/- mice Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.36 0.002
-
 benzaldehyde
-
 Homo sapiens
1.2.1.36 0.085
-
 NAD+ partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 0.1 mM 3-deoxyglucosone Homo sapiens
1.2.1.36 0.095
-
 3-deoxyglucosone partially purified recombinant ALDH1A1 at pH 7.1 and in the presence of 1 mM NAD Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.36 55000
-
 sequence analysis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.36 Homo sapiens
-
-
-
1.2.1.36 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.36 by gel filtration, more than 400fold with a yield of about 1%, to near homogeneity Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.36 erythrocyte
-
 Mus musculus
-
1.2.1.36 erythrocyte
-
 Homo sapiens
-
1.2.1.36 liver highest activity Mus musculus
-
1.2.1.36 lung intermediate activity Mus musculus
-
1.2.1.36 additional information no activity is detectable in brain, thymus, spleen, kidneys, skeletal muscle and heart Mus musculus
-
1.2.1.36 testis intermediate activity Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.36 3-deoxyglucosone + NAD+ + H2O
-
 Mus musculus 2-keto-3-deoxygluconate + NADH + H+
-
 ?
1.2.1.36 3-deoxyglucosone + NAD+ + H2O
-
 Homo sapiens 2-keto-3-deoxygluconate + NADH + H+
-
 ?
1.2.1.36 benzaldehyde + NAD+ + H2O
-
 Homo sapiens benzoate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.36 aldehyde dehydrogenase 1A1
-
 Mus musculus
1.2.1.36 aldehyde dehydrogenase 1A1
-
 Homo sapiens
1.2.1.36 ALDH1A1
-
 Mus musculus
1.2.1.36 ALDH1A1
-
 Homo sapiens
1.2.1.36 retinaldehyde dehydrogenase 1
-
 Mus musculus
1.2.1.36 retinaldehyde dehydrogenase 1
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.36 7.1 9
-
 Homo sapiens