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Literature summary extracted from

  • Zees, A.C.; Pyrpassopoulos, S.; Vorgias, C.E.
    Insights into the role of the (alpha+beta) insertion in the TIM-barrel catalytic domain, regarding the stability and the enzymatic activity of chitinase A from Serratia marcescens (2009), Biochim. Biophys. Acta, 1794, 23-31.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.14 crystal stucture analysis, overview Serratia marcescens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 additional information an alpha + beta insertion between the B7 beta-strand and A7 alpha-helix of the TIM-barrel, an FnIII domain at the N-terminus of the molecule and a hinge region that connects the latter to the catalytic domain. Structure modelling of the (alpha + beta)DELTAChiA mutant, overview Serratia marcescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
additional information kinetics of wild-type and alpha/beta domain deletion mutant, overview Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Serratia marcescens P07254
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAc + H2O
-
Serratia marcescens ?
-
?
3.2.1.14 4-methylumbelliferyl GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
Serratia marcescens ?
-
?
3.2.1.14 chitin + H2O
-
Serratia marcescens ?
-
?
3.2.1.14 additional information the enzyme is also active with chitooligosachharides from GlnNAc to GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAcbeta(1-4)GlnNAc, overview Serratia marcescens ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 More the catalytic domain has a TIM-barrel fold, structure modelling, overview Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 ChiA
-
Serratia marcescens
3.2.1.14 chitinase A
-
Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 25 45 depending on the pH, overview Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 25 45
-
Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 60
-
ChiA exhibits an endothermic irreversible transition at an apparent Tm of 60.7°C Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
at 58°C Serratia marcescens
3.2.1.14 6.5
-
at 42°C Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 9
-
Serratia marcescens