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Literature summary extracted from

  • Fukamizo, T.; Miyake, R.; Tamura, A.; Ohnuma, T.; Skriver, K.; Pursiainen, N.V.; Juffer, A.H.
    A flexible loop controlling the enzymatic activity and specificity in a glycosyl hydrolase family 19 endochitinase from barley seeds (Hordeum vulgare L.) (2009), Biochim. Biophys. Acta, 1794, 1159-1167.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 W72A the mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme Hordeum vulgare
3.2.1.14 W72A/W82A the double mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme, but the effect lies between the affection of the individual mutations Hordeum vulgare
3.2.1.14 W82A the mutation affects the thermal stability and specific activities toward glycol chitin and chitin hexasaccharide compared to the wild-type enzyme Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Hordeum vulgare P23951
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 seed
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 additional information
-
 no activity of mutant W72A/W82A with substrate GlcNAc hexasaccharide Hordeum vulgare
3.2.1.14 0.02
-
 mutant W82A, substrate glycol chitin Hordeum vulgare
3.2.1.14 0.38
-
 mutant W72A/W82A, substrate glycol chitin Hordeum vulgare
3.2.1.14 2.53
-
 mutant W72A, substrate glycol chitin Hordeum vulgare
3.2.1.14 6.31
-
 wild-type enzyme, substrate glycol chitin Hordeum vulgare
3.2.1.14 23.4
-
 mutant W82A, substrate GlcNAc hexasaccharide Hordeum vulgare
3.2.1.14 139
-
 wild-type enzyme, substrate GlcNAc hexasaccharide Hordeum vulgare
3.2.1.14 251
-
 mutant W72A, substrate GlcNAc hexasaccharide Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O a flexible loop controlling the enzymatic activity and specificity in the glycosyl hydrolase family 19 endochitinase from barley seeds Hordeum vulgare ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?
3.2.1.14 GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
-
 Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 More molecular dynamics simulations of wild-type and mutant enzymes, overview Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 endochitinase
-
 Hordeum vulgare
3.2.1.14 More the endochitinase belongs to the glycosyl hydrolase family 19 Hordeum vulgare

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 25 40 assay at Hordeum vulgare