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Literature summary extracted from
- Simultaneous refolding and purification of a recombinant lipase with an intein tag by affinity precipitation with chitosan (2008), Biochim. Biophys. Acta, 1784, 1825-1829.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | expressed in Escherichia coli strain ER2566 as recombinant enzyme with intein tag | Burkholderia cepacia |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.3 | 0.11 | - |
4-nitrophenyl palmitate | native enzyme, at pH 7 and 37°C | Burkholderia cepacia |  |
| 3.1.1.3 | 0.12 | - |
4-nitrophenyl palmitate | recombinant enzyme, at pH 7 and 37°C | Burkholderia cepacia | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 29000 | - |
recombinant enzyme, SDS-PAGE | Burkholderia cepacia |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Burkholderia cepacia | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | chitin column chromatography and chitosan affinity precipitation (0.3 (w/v) chitosan is required for obtaining maximum active enzyme) | Burkholderia cepacia |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | the intein tag is cleaved with dithiothreitol and the refolded lipase is obtained in active form (activity recovery of 80%) | Burkholderia cepacia |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 2960 | - |
refolded and purified enzyme, at pH 7 and 37°C | Burkholderia cepacia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | 4-nitrophenyl palmitate + H2O | - |
Burkholderia cepacia | 4-nitrophenol + palmitate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | lipase | - |
Burkholderia cepacia |
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Release 2023.1 (January 2023)
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