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Literature summary extracted from

  • Li, L.; Patterson, D.P.; Fox, C.C.; Lin, B.; Coschigano, P.W.; Marsh, E.N.
    Subunit structure of benzylsuccinate synthase (dagger) (2009), Biochemistry, 48, 1284-1292.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.11 expression in Escherichia coli BL21DE3 cells, the vectors pET-28b, pACYC-Duet, pET-Duet and pRSF-Duet are used Thauera aromatica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.11 7000
-
 gamma subunit, determined by SDS-PAGE Thauera aromatica
4.1.99.11 14000
-
 beta subunit, determined by SDS-PAGE Thauera aromatica
4.1.99.11 100000
-
 alpha subunit, determined by SDS-PAGE Thauera aromatica
4.1.99.11 230000
-
 determined by gel filtration, consistent with an alpha2beta2gamma2 subunit structure Thauera aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.11 toluene + fumarate Thauera aromatica
-
 benzylsuccinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.11 Thauera aromatica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.11 using a Ni-NTA-agarose column Thauera aromatica

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.1.99.11 the alpha subunit is purified and refolded from inclusion bodies Thauera aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.11 toluene + fumarate
-
 Thauera aromatica benzylsuccinate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.11 heterotrimer alpha, beta and gamma subunit Thauera aromatica
4.1.99.11 hexamer the structural model for benzylsuccinate synthase proposes an alpha2beta2gamma2-complex Thauera aromatica

Synonyms

EC Number Synonyms Comment Organism
4.1.99.11 benzylsuccinate synthase
-
 Thauera aromatica
4.1.99.11 BSS
-
 Thauera aromatica

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.11 iron-sulfur centre
-
 Thauera aromatica