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Literature summary extracted from

  • Brandt, G.S.; Nemeria, N.; Chakraborty, S.; McLeish, M.J.; Yep, A.; Kenyon, G.L.; Petsko, G.A.; Jordan, F.; Ringe, D.
    Probing the active center of benzaldehyde lyase with substitutions and the pseudosubstrate analogue benzoylphosphonic acid methyl ester (2008), Biochemistry, 47, 7734-7743.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.2.38 synthesis the enzyme is a tool in chemoenzymatic synthesis of chiral alpha-hydroxyketones Pseudomonas fluorescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.38 the plasmid pKK-BAL-His is constructed for expression of wild-type BAL in Escherichia coli BL21DE3pLysS cells Pseudomonas fluorescens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.38 the structure of benzaldehyde lyase in its covalent complex with methyl benzoylphosphonate is determined to 2.49 A Pseudomonas fluorescens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.38 H29A the role of His29 in cofactor activation and catalysis is delineated Pseudomonas fluorescens
4.1.2.38 W163A the role of Trp163 in cofactor activation and catalysis is delineated Pseudomonas fluorescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.38 Mg2+
-
 Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.38 2-hydroxy-1,2-diphenylethanone Pseudomonas fluorescens
-
 benzaldehyde
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.38 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.38
-
 Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.38 2-hydroxy-1,2-diphenylethanone
-
 Pseudomonas fluorescens benzaldehyde
-
 r
4.1.2.38 methyl benzoylphosphonate
-
 Pseudomonas fluorescens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.38 homotetramer
-
 Pseudomonas fluorescens

Synonyms

EC Number Synonyms Comment Organism
4.1.2.38 BAL
-
 Pseudomonas fluorescens
4.1.2.38 benzaldehyde lyase
-
 Pseudomonas fluorescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.2.38 30
-
 activity assay Pseudomonas fluorescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.38 8
-
 activity assay Pseudomonas fluorescens

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.38 thiamine diphosphate
-
 Pseudomonas fluorescens