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Literature summary extracted from

  • Day, J.P.; Cleghon, V.; Houslay, M.D.; Davies, S.A.
    Regulation of a Drosophila melanogaster cGMP-specific phosphodiesterase by prenylation and interaction with a prenyl-binding protein (2008), Biochem. J., 414, 363-374.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.35 C1128S the unprenylated mutant enzyme is mainly localized to the cytosol Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.35 sildenafil
-
 Drosophila melanogaster
3.1.4.35 zaprinast
-
 Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.35 0.0281
-
 3',5'-cAMP wild type enzyme, in 50 mM KCl, 10 mM EGTA, 1.92 mM MgCl2, 1 mM dithiothreitol, and 50 mM HEPES, pH 7.4 Drosophila melanogaster
3.1.4.35 0.0354
-
 3',5'-cAMP mutant enzyme C1128S, in 50 mM KCl, 10 mM EGTA, 1.92 mM MgCl2, 1 mM dithiothreitol, and 50 mM HEPES, pH 7.4 Drosophila melanogaster

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.35 cytosol C1128S-PDE5/6 mutant form is located primarily in the cytosol, although some residual association occurs at the apical membrane Drosophila melanogaster 5829
-
3.1.4.35 plasma membrane PDE5/6 is exclusively associated with the apical membrane Drosophila melanogaster 5886
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.35 130000
-
 SDS-PAGE Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.35 additional information Drosophila melanogaster prenylation of the enzyme is required for plasma-membrane targeting ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.35 Drosophila melanogaster
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.35 S2 cell
-
 Drosophila melanogaster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.35 3',5'-cAMP + H2O
-
 Drosophila melanogaster 5'-AMP
-
 ?
3.1.4.35 3',5'-cGMP + H2O
-
 Drosophila melanogaster 5'-GMP
-
 ?
3.1.4.35 additional information prenylation of the enzyme is required for plasma-membrane targeting Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.35 cGMP-PDE
-
 Drosophila melanogaster
3.1.4.35 cGMP-specific phosphodiesterase
-
 Drosophila melanogaster
3.1.4.35 PDE5/6
-
 Drosophila melanogaster