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Literature summary extracted from

  • Rahier, A.; Pierre, S.; Riveill, G.; Karst, F.
    Identification of essential amino acid residues in a sterol 8,7-isomerase from Zea mays reveals functional homology and diversity with the isomerases of animal and fungal origin (2008), Biochem. J., 414, 247-259.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.3.5 expressed in the Saccharomyces cerevisiae mutant erg2 Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
5.3.3.5 D107V inactive Zea mays
5.3.3.5 E116V mutant exhibits 72% of wild type activity Saccharomyces cerevisiae
5.3.3.5 E121V inactive Zea mays
5.3.3.5 E73V mutant exhibits 100% of wild type activity Saccharomyces cerevisiae
5.3.3.5 E78V inactive Zea mays
5.3.3.5 H69L mutant exhibits 70% of wild type activity Saccharomyces cerevisiae
5.3.3.5 H74L inactive Zea mays
5.3.3.5 T119I inactive Saccharomyces cerevisiae
5.3.3.5 T124I mutant exhibits 10% of wild type activity Zea mays
5.3.3.5 W193L inactive Zea mays
5.3.3.5 W66L inactive Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.3.5 AY9944
-
 Zea mays
5.3.3.5 fenpropimorph
-
 Saccharomyces cerevisiae
5.3.3.5 fenpropimorph
-
 Zea mays
5.3.3.5 additional information not inhibited by N-benzamido-decalin, tamoxifen, N-benzyl-8-azadecalin, and AY9944 Saccharomyces cerevisiae
5.3.3.5 additional information not inhibited by N-benzamido-decalin Zea mays
5.3.3.5 SR31747
-
 Saccharomyces cerevisiae
5.3.3.5 SR31747
-
 Zea mays
5.3.3.5 tamoxifen
-
 Zea mays
5.3.3.5 tridemorph
-
 Saccharomyces cerevisiae
5.3.3.5 tridemorph
-
 Zea mays
5.3.3.5 Trifluoperazine
-
 Saccharomyces cerevisiae
5.3.3.5 Trifluoperazine
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.3.5 0.116
-
 stigmasta-8-en-3beta-ol
-
 Zea mays
5.3.3.5 0.512
-
 cholesta-8,24-dien-3beta-ol
-
 Zea mays
5.3.3.5 0.522
-
 4alpha-methyl-ergosta-8,24-dien-3beta-ol
-
 Zea mays

Organism

EC Number Organism UniProt Comment Textmining
5.3.3.5 Saccharomyces cerevisiae
-
-
-
5.3.3.5 Zea mays
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.3.5 4alpha-methyl-ergosta-8,24-dien-3beta-ol
-
 Zea mays ?
-
 ?
5.3.3.5 4alpha-methyl-fecosterol
-
 Zea mays 4alpha-methyl-ergosta-7,24-dien-3beta-ol
-
 ?
5.3.3.5 cholesta-8,24-dien-3beta-ol
-
 Zea mays ?
-
 ?
5.3.3.5 DELTA8-sitostenol
-
 Zea mays stigmast-7-en-3beta-ol
-
 ?
5.3.3.5 additional information 4,4-dimethyl-cholesta-8,24-dien-3beta-ol, 4,4-dimethyl-ergosta-8,24-dien-3beta-ol, 4alpha,14aloha-dimethyl-ergosta-8,24-dien-3beta-ol, and cholest-8(14)-en-3beta-ol are no substrates Zea mays ?
-
 ?
5.3.3.5 stigmasta-8-en-3beta-ol
-
 Zea mays ?
-
 ?
5.3.3.5 zymosterol
-
 Saccharomyces cerevisiae cholesta-7,24-dienol
-
 ?
5.3.3.5 zymosterol
-
 Zea mays cholesta-7,24-dienol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.3.5 8,7SI
-
 Saccharomyces cerevisiae
5.3.3.5 8,7SI
-
 Zea mays
5.3.3.5 EBP
-
 Zea mays
5.3.3.5 ERG2p
-
 Saccharomyces cerevisiae
5.3.3.5 sterol 8,7-isomerase
-
 Saccharomyces cerevisiae
5.3.3.5 sterol 8,7-isomerase
-
 Zea mays

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.3.3.5 0.00003
-
-
 Saccharomyces cerevisiae fenpropimorph
5.3.3.5 0.0001
-
-
 Zea mays AY9944
5.3.3.5 0.0001
-
-
 Zea mays SR31747
5.3.3.5 0.00021
-
-
 Zea mays fenpropimorph
5.3.3.5 0.0006
-
-
 Saccharomyces cerevisiae SR31747
5.3.3.5 0.00085
-
-
 Zea mays tridemorph
5.3.3.5 0.001
-
-
 Zea mays Trifluoperazine
5.3.3.5 0.001
-
-
 Saccharomyces cerevisiae tridemorph
5.3.3.5 0.0085
-
-
 Zea mays tamoxifen
5.3.3.5 0.5
-
 IC50 above 0.5 mM Saccharomyces cerevisiae Trifluoperazine