EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.8.11 | from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP for introduction of C-terminal EYFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections | Arabidopsis thaliana |
2.7.8.11 | from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP or -CFP for introduction of C-terminal EYFP- or CFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.8.11 | C148W | polymorphism of unclear relevance, amplified from plant cDNA, mismatch with annotated genomic sequence | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.8.11 | endoplasmic reticulum | transient gene expression in onion epidermal cells, C-terminal CFP-tag, confocal microscopy, centre of punctuate structures | Arabidopsis thaliana | 5783 | - |
2.7.8.11 | endoplasmic reticulum | transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, periphery of punctuate structures | Arabidopsis thaliana | 5783 | - |
2.7.8.11 | Golgi apparatus | transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, limited overlap with marker (rat sialyl-S-transferase) | Arabidopsis thaliana | 5794 | - |
2.7.8.11 | membrane | membrane fraction of overexpressing Escherichia coli | Arabidopsis thaliana | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.11 | CDP-diacylglycerol + D-myo-inositol | Arabidopsis thaliana | key step in lipid biosynthesis | CMP + phosphatidyl-1D-myo-inositol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.8.11 | Arabidopsis thaliana | Q8GUK6 | ecotype Columbia-0 | - |
2.7.8.11 | Arabidopsis thaliana | Q8LBA6 | ecotype Columbia-0 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.8.11 | as bacterial membrane pellets, no further specific purification possible | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.8.11 | leaf | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.8.11 | CDP-diacylglycerol + D-myo-inositol | key step in lipid biosynthesis | Arabidopsis thaliana | CMP + phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-diacylglycerol + D-myo-inositol | D-myo-inositol substrates: phosphatidylethanolamine, phosphatidic acid, overexpressed enzyme in bacterial membrane pellets, 30 min, 22°C, pH 8, 1% (v/v) Triton X-100, 20 mM MgCl2 | Arabidopsis thaliana | CMP + phosphatidyl-1D-myo-inositol | fatty acid analysis by thin-layer chromatography | ? | |
2.7.8.11 | CDP-diheptadecanoylglycerol + D-myo-inositol | - |
Arabidopsis thaliana | CMP + 1,2-diheptadecanoyl-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-dilinoleoylglycerol + D-myo-inositol | - |
Arabidopsis thaliana | CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-dilinoleoylglycerol + D-myo-inositol | highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dioleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol | Arabidopsis thaliana | CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-dioleoylglycerol + D-myo-inositol | - |
Arabidopsis thaliana | CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-dioleoylglycerol + D-myo-inositol | highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dilinoleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol | Arabidopsis thaliana | CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | CDP-dipalmitoylglycerol + D-myo-inositol | - |
Arabidopsis thaliana | CMP + 1,2-dipalmitoyl-phosphatidyl-1D-myo-inositol | - |
? | |
2.7.8.11 | additional information | highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid | Arabidopsis thaliana | ? | - |
? | |
2.7.8.11 | additional information | in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants | Arabidopsis thaliana | ? | - |
? | |
2.7.8.11 | additional information | in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants | Arabidopsis thaliana | ? | - |
? | |
2.7.8.11 | additional information | moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid | Arabidopsis thaliana | ? | - |
? | |
2.7.8.11 | additional information | preference of C18-fatty acid CDP-diacylglycerol substrates | Arabidopsis thaliana | ? | - |
? | |
2.7.8.11 | additional information | preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates | Arabidopsis thaliana | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.8.11 | phosphatidyinositol synthase | - |
Arabidopsis thaliana |
2.7.8.11 | PIS1 | - |
Arabidopsis thaliana |
2.7.8.11 | PIS1 | gene locus At1g68000 | Arabidopsis thaliana |
2.7.8.11 | PIS2 | - |
Arabidopsis thaliana |
2.7.8.11 | PIS2 | gene locus At4g38570 | Arabidopsis thaliana |
2.7.8.11 | PtdIns synthase | - |
Arabidopsis thaliana |