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Literature summary extracted from

  • Loefke, C.; Ischebeck, T.; Koenig, S.; Freitag, S.; Heilmann, I.
    Alternative metabolic fates of phosphatidylinositol produced by phosphatidylinositol synthase isoforms in Arabidopsis thaliana (2008), Biochem. J., 413, 115-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.11 from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP for introduction of C-terminal EYFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections Arabidopsis thaliana
2.7.8.11 from flower and silique cDNA, in pGEM-T-Easy for sequencing, in pET28b for inducible expression in Escherichia coli C43(DE3), in pUC18-ENTR-EYFP or -CFP for introduction of C-terminal EYFP- or CFP-tag followed by subcloning into pCAMBIA3300-0GC for Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliania plants or transient expression in onion (Allium cepa) epidermal sections Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.11 C148W polymorphism of unclear relevance, amplified from plant cDNA, mismatch with annotated genomic sequence Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.11 endoplasmic reticulum transient gene expression in onion epidermal cells, C-terminal CFP-tag, confocal microscopy, centre of punctuate structures Arabidopsis thaliana 5783
-
2.7.8.11 endoplasmic reticulum transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, periphery of punctuate structures Arabidopsis thaliana 5783
-
2.7.8.11 Golgi apparatus transient gene expression in onion epidermal cells, C-terminal EYFP-tag, confocal microscopy, limited overlap with marker (rat sialyl-S-transferase) Arabidopsis thaliana 5794
-
2.7.8.11 membrane membrane fraction of overexpressing Escherichia coli Arabidopsis thaliana 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + D-myo-inositol Arabidopsis thaliana key step in lipid biosynthesis CMP + phosphatidyl-1D-myo-inositol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.11 Arabidopsis thaliana Q8GUK6 ecotype Columbia-0
-
2.7.8.11 Arabidopsis thaliana Q8LBA6 ecotype Columbia-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.11 as bacterial membrane pellets, no further specific purification possible Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.11 leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + D-myo-inositol key step in lipid biosynthesis Arabidopsis thaliana CMP + phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 CDP-diacylglycerol + D-myo-inositol D-myo-inositol substrates: phosphatidylethanolamine, phosphatidic acid, overexpressed enzyme in bacterial membrane pellets, 30 min, 22°C, pH 8, 1% (v/v) Triton X-100, 20 mM MgCl2 Arabidopsis thaliana CMP + phosphatidyl-1D-myo-inositol fatty acid analysis by thin-layer chromatography ?
2.7.8.11 CDP-diheptadecanoylglycerol + D-myo-inositol
-
Arabidopsis thaliana CMP + 1,2-diheptadecanoyl-phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 CDP-dilinoleoylglycerol + D-myo-inositol
-
Arabidopsis thaliana CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 CDP-dilinoleoylglycerol + D-myo-inositol highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dioleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol Arabidopsis thaliana CMP + 1,2-dilinoleoyl-phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 CDP-dioleoylglycerol + D-myo-inositol
-
Arabidopsis thaliana CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 CDP-dioleoylglycerol + D-myo-inositol highest activity, preferred in competition assay additionally containing 1,2-dipalmitoyl-, 1,2-dilinoleoyl-, and 1,2-diheptadecanoyl-CDP-diacylglycerol Arabidopsis thaliana CMP + 1,2-dioleoyl-phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 CDP-dipalmitoylglycerol + D-myo-inositol
-
Arabidopsis thaliana CMP + 1,2-dipalmitoyl-phosphatidyl-1D-myo-inositol
-
?
2.7.8.11 additional information highly efficient conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic, acid linoleic acid and linolenic acid, complete conversion of CDP-diacylglycerol residues containing palmitic, acid linoleic acid and linolenic acid Arabidopsis thaliana ?
-
?
2.7.8.11 additional information in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, and polyphosphoinositide (PtdIns(4,5)P, PtdIns(4,5)P2) that are enriched in polyunsaturated fatty acids compared to wild-type plants Arabidopsis thaliana ?
-
?
2.7.8.11 additional information in transgenic plants, elevated formation of phosphatidic acid, phosphatidylinositol, phosphatidylethanolamine and diacylglycerol containing less polyunsaturated fatty acids but more saturated and monounsaturated fatty acids, no change in fatty acid composition of polyphopshoinositides compared to wild-type plants Arabidopsis thaliana ?
-
?
2.7.8.11 additional information moderate conversion of biological mixture of CDP-diacylglycerol substrates (synthesised from phosphatidylethanolamine in plant leaves) into phosphatidyl-1D-myo-inositol mainly containing palmitic acid and little linoleic acid, non-converted CDP-diacylglycerol residues containing linoleic acid and linolenic acid Arabidopsis thaliana ?
-
?
2.7.8.11 additional information preference of C18-fatty acid CDP-diacylglycerol substrates Arabidopsis thaliana ?
-
?
2.7.8.11 additional information preference of polyansaturated C18-fatty acid CDP-diacylglycerol substrates Arabidopsis thaliana ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.11 phosphatidyinositol synthase
-
Arabidopsis thaliana
2.7.8.11 PIS1
-
Arabidopsis thaliana
2.7.8.11 PIS1 gene locus At1g68000 Arabidopsis thaliana
2.7.8.11 PIS2
-
Arabidopsis thaliana
2.7.8.11 PIS2 gene locus At4g38570 Arabidopsis thaliana
2.7.8.11 PtdIns synthase
-
Arabidopsis thaliana