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Literature summary extracted from

  • Müller, S.; Walter, R.D.
    Purification and characterization of polyamine oxidase from Ascaris suum (1992), Biochem. J., 1283, 75-80.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.16 dithioerythritol up to 0.010 mM increase the enzyme activity, higher concentrations inhibited it Ascaris suum
1.5.3.16 dithiothreitol up to 0.010 mM increase the enzyme activity, higher concentrations inhibited it Ascaris suum
1.5.3.16 Iproniazid 0.01 mM, 87% inhibition Ascaris suum
1.5.3.16 isoniazid 0.01 mM, 81% inhibition Ascaris suum
1.5.3.16 MDL 27391
-
 Ascaris suum
1.5.3.16 MDL 27695
-
 Ascaris suum
1.5.3.16 additional information the polyamine oxidase inhibitor MDL 72527 has no effect on the parasite polyamine oxidase activity Ascaris suum
1.5.3.16 NEM 0.01 mM, 85% inhibition Ascaris suum
1.5.3.17 dithioerythritol up to 0.010 mM increase the enzyme activity, higher concentrations inhibit Ascaris suum
1.5.3.17 dithiothreitol up to 0.010 mM increase the enzyme activity, higher concentrations inhibit Ascaris suum
1.5.3.17 Iproniazid 0.01 mM, 87% inhibition Ascaris suum
1.5.3.17 isoniazid 0.01 mM, 81% inhibition Ascaris suum
1.5.3.17 MDL 27391
-
 Ascaris suum
1.5.3.17 MDL 27695
-
 Ascaris suum
1.5.3.17 additional information the polyamine oxidase inhibitor MDL 72527 has no effect on the parasite polyamine oxidase activity Ascaris suum
1.5.3.17 NEM 0.01 mM, 85% inhibition Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.16 0.35
-
 spermine pH 8.5, 37°C Ascaris suum
1.5.3.16 0.66
-
 spermidine pH 8.5, 37°C Ascaris suum
1.5.3.16 1.6
-
 benzylamidine pH 8.5, 37°C Ascaris suum
1.5.3.16 2
-
 norspermine pH 8.5, 37°C Ascaris suum
1.5.3.16 2.5
-
 tryptamine pH 8.5, 37°C Ascaris suum
1.5.3.16 5
-
 norspermidine pH 8.5, 37°C Ascaris suum
1.5.3.17 0.35
-
 spermine pH 8.5, 37°C Ascaris suum
1.5.3.17 0.66
-
 spermidine pH 8.5, 37°C Ascaris suum
1.5.3.17 1.6
-
 benzylamidine pH 8.5, 37°C Ascaris suum
1.5.3.17 2
-
 norspermine pH 8.5, 37°C Ascaris suum
1.5.3.17 2.5
-
 tryptamine pH 8.5, 37°C Ascaris suum
1.5.3.17 5
-
 norspermidine pH 8.5, 37°C Ascaris suum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.3.16 additional information copper and iron are not cofactors Ascaris suum
1.5.3.17 additional information copper and iron are not cofactors Ascaris suum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.16 66000
-
 x * 66000, SDS-PAGE Ascaris suum
1.5.3.17 66000
-
 x * 66000, SDS-PAGE Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.16 Ascaris suum
-
-
-
1.5.3.17 Ascaris suum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.16
-
 Ascaris suum
1.5.3.17
-
 Ascaris suum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.3.16 2.3
-
-
 Ascaris suum
1.5.3.17 2.3
-
-
 Ascaris suum

Storage Stability

EC Number Storage Stability Organism
1.5.3.16 -20°C, stable for 2 days minimal loss of activity, complete loss of enzyme activity after 14 days Ascaris suum
1.5.3.17 -20°C, stable for 2 days (minimal loss of activity), complete loss of enzyme activity after 14 days Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.16 benzylamidine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.16 additional information N-acetylated polyamines and diamines, e.g. Nl-acetylspermidine, N8-acetylspermidine, N-acetylspermine, N1,N12-diacetylspermine, putrescine, cadaverine and histamine, are not accepted as substrates. Bis(benzyl)polyamines, such as MDL 27695 and MDL 27391, are not substrates Ascaris suum ?
-
 ?
1.5.3.16 norspermidine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.16 norspermine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.16 spermidine + O2 + H2O
-
 Ascaris suum putrescine + 3-aminopropanal + H2O2
-
 ?
1.5.3.16 spermine + O2 + H2O
-
 Ascaris suum spermidine + 3-aminopropanal + H2O2
-
 ?
1.5.3.16 tryptamine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.17 benzylamidine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.17 additional information N-acetylated polyamines and diamines, e.g. N1-acetylspermidine, N8-acetylspermidine, N-acetylspermine, N1,N12-diacetylspermine, putrescine, cadaverine and histamine, are not accepted as substrates. Bis(benzyl)polyamines, such as MDL 27695 and MDL 27391, are not substrates Ascaris suum ?
-
 ?
1.5.3.17 norspermidine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.17 norspermine + O2 + H2O
-
 Ascaris suum ?
-
 ?
1.5.3.17 spermidine + O2 + H2O
-
 Ascaris suum putrescine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 spermine + O2 + H2O
-
 Ascaris suum spermidine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 tryptamine + O2 + H2O
-
 Ascaris suum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.16 ? x * 66000, SDS-PAGE Ascaris suum
1.5.3.17 ? x * 66000, SDS-PAGE Ascaris suum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.16 30
-
 complete loss of activity after 60 min Ascaris suum
1.5.3.16 37
-
 complete loss of activity after 30 min Ascaris suum
1.5.3.16 42
-
 complete loss of activity after 20 min Ascaris suum
1.5.3.16 56
-
 complete loss of activity after 10 min Ascaris suum
1.5.3.17 30
-
 60 min, complete loss of activity Ascaris suum
1.5.3.17 37
-
 60 min, complete loss of activity Ascaris suum
1.5.3.17 42
-
 60 min, complete loss of activity Ascaris suum
1.5.3.17 56
-
 60 min, complete loss of activity Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.16 8.5
-
-
 Ascaris suum
1.5.3.17 8.5
-
-
 Ascaris suum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.16 FAD
-
 Ascaris suum
1.5.3.17 FAD
-
 Ascaris suum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.3.16 0.02
-
 MDL 27695 pH 8.5, 37°C Ascaris suum
1.5.3.17 0.02
-
 MDL 27695 pH 8.5, 37°C Ascaris suum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.3.16 Ascaris suum isoelectric focusing
-
 7.8
1.5.3.17 Ascaris suum isoelectric focusing
-
 7.8