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Literature summary extracted from

  • Liu, Z.; Chi, Z.; Wang, L.; Li, J.
    Production, purification and characterization of an extracellular lipase from Aureobasidium pullulans HN2.3 with potential application for the hydrolysis of edible oils (2008), Biochem. Eng. J., 40, 445-451.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ag+ 80% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Ba2+ 74.3% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Ca2+ 73% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Co2+ 68% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Cu2+ 62% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Fe2+ 30% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Fe3+ 77% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Hg2+ 32.3% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 iodoacetic acid 88% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Li+ 82% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Mg2+ 78% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Mn2+ 71% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 additional information not inhibited by EDTA Aureobasidium pullulans
3.1.1.3 phenylmethanesulfonyl fluoride 67.2% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 SDS 21.3% residual activity at 5 mM Aureobasidium pullulans
3.1.1.3 Zn2+ 59.1% residual activity at 5 mM Aureobasidium pullulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 0.608
-
 4-nitrophenyl laurate
-
 Aureobasidium pullulans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Aureobasidium pullulans
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 63500
-
 SDS-PAGE Aureobasidium pullulans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Aureobasidium pullulans
-
-
-
3.1.1.3 Aureobasidium pullulans HN2.3
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 ammonium sulfate precipitation, Sephadex-75 gel filtration, and DEAE-Sepharose column chromatography Aureobasidium pullulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 26
-
 enzyme from crude supernatant Aureobasidium pullulans
3.1.1.3 88.6
-
 enzyme after 3.4fold purification Aureobasidium pullulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Aureobasidium pullulans 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Aureobasidium pullulans HN2.3 4-nitrophenol + laurate
-
 ?
3.1.1.3 lard + H2O
-
 Aureobasidium pullulans ?
-
 ?
3.1.1.3 lard + H2O
-
 Aureobasidium pullulans HN2.3 ?
-
 ?
3.1.1.3 olive oil + H2O
-
 Aureobasidium pullulans ?
-
 ?
3.1.1.3 olive oil + H2O
-
 Aureobasidium pullulans HN2.3 ?
-
 ?
3.1.1.3 peanut oil + H2O highest hydrolytic activity towards peanut oil Aureobasidium pullulans ?
-
 ?
3.1.1.3 peanut oil + H2O highest hydrolytic activity towards peanut oil Aureobasidium pullulans HN2.3 ?
-
 ?
3.1.1.3 soybean oil + H2O
-
 Aureobasidium pullulans ?
-
 ?
3.1.1.3 soybean oil + H2O
-
 Aureobasidium pullulans HN2.3 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 extracellular lipase
-
 Aureobasidium pullulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 35
-
-
 Aureobasidium pullulans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 25 50
-
 Aureobasidium pullulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 20 50 the residual lipase activity is maintained at 100% and 71% activity compared to the control after treatment for 1 h at 20°C and 30°C, respectively, the enzyme is inactivated rapidly at temperature higher than 20°C and is inactivated totally at 50°C within 1 h Aureobasidium pullulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8.5
-
-
 Aureobasidium pullulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.3 4 8.5
-
 Aureobasidium pullulans