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Literature summary extracted from

  • Bazan, S.; Issoglio, F.M.; Carrizo, M.E.; Curtino, J.A.
    The intramolecular autoglucosylation of monomeric glycogenin (2008), Biochem. Biophys. Res. Commun., 371, 328-332.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.186 preparation of a truncated already glucosylated His-tag fusion protein in Escherichia coli ER2566, expression of the truncated non-glucosylated His-tag fusion protein in Escherichia coli CGSC 4997 Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.186 34000
-
 non-glycosylated truncated protein, gel filtration of a 0.0005 mM solution Oryctolagus cuniculus
2.4.1.186 38000
-
 1 * 38000, native enzyme, 50-70% of the activity of the dimer Oryctolagus cuniculus
2.4.1.186 38000
-
 2 * 38000, native enzyme Oryctolagus cuniculus
2.4.1.186 59000
-
 non-glycosylated truncated protein, gel filtration of a 0.02 mM solution Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.186 UDP-glucose + glycogenin Oryctolagus cuniculus essential for the formation of glycogen granules, binds a chain of 5-13 glucose molecules at a specific tyrosine residue by autoglycosylation, catalyzes two chemically different autoglucosylation reactions, the glucosylation of a tyrosine hydroxyl group and the formation of alpha-1,4 glucosidic linkages by subsequent glucosylations UDP + glucosylated glycogenin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.186 Oryctolagus cuniculus P13280
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.186 glycoprotein autoglycosylation Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.186 recombinant proteins Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.186 muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.186 UDP-glucose + glycogenin
-
 Oryctolagus cuniculus UDP + glucosylated glycogenin
-
 ?
2.4.1.186 UDP-glucose + glycogenin essential for the formation of glycogen granules, binds a chain of 5-13 glucose molecules at a specific tyrosine residue by autoglycosylation, catalyzes two chemically different autoglucosylation reactions, the glucosylation of a tyrosine hydroxyl group and the formation of alpha-1,4 glucosidic linkages by subsequent glucosylations Oryctolagus cuniculus UDP + glucosylated glycogenin
-
 ?
2.4.1.186 UDP-xylose + glycogenin
-
 Oryctolagus cuniculus UDP + xylosylated glycogenin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.186 dimer 2 * 34000, non-glycosylated truncated protein, 0.02 mM enzyme solution Oryctolagus cuniculus
2.4.1.186 dimer 2 * 38000, native enzyme Oryctolagus cuniculus
2.4.1.186 monomer 1 * 34000, non-glycosylated truncated protein, 0.0005 mM enzyme solution Oryctolagus cuniculus
2.4.1.186 monomer 1 * 38000, native enzyme, 50-70% of the activity of the dimer Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
2.4.1.186 glycogenin
-
 Oryctolagus cuniculus