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Literature summary extracted from

  • Roy, S.; Choudhury, S.R.; Sengupta, D.N.
    Analysis of processivity of mungbean dideoxynucleotide-sensitive DNA polymerase and detection of the activity and expression of the enzyme in the meristematic and meiotic tissues and following DNA damaging agent (2008), Arch. Biochem. Biophys., 475, 55-65.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.7 ATP considerable enhancement in processivity at 1 mM ATP Vigna radiata
2.7.7.7 spermidine considerable enhancement in processivity at 2-4 mM spermidine Vigna radiata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.7 ddTTP processivity is strongly inhibited in the presence of 0.01 and 0.02 mM ddTTP Vigna radiata
2.7.7.7 Mg2+ Mg2+ concentrations beyond 6 mM are inhibitory in activity assay Vigna radiata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 K+ optimal processivity at 100-150 mM Vigna radiata
2.7.7.7 Mg2+ optimal processivity at 6 mM, no synthesis is observed in the absence of Mg2+ Vigna radiata
2.7.7.7 Mn2+
-
 Vigna radiata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 62000
-
 SDS-PAGE Vigna radiata

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Vigna radiata
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.7 leaf
-
 Vigna radiata
-
2.7.7.7 seedling active in both meristematic and meiotic tissues Vigna radiata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn
-
 Vigna radiata diphosphate + DNAn+1
-
 ?
2.7.7.7 additional information the enzyme is unable to use single stranded DNA or double stranded blunt end DNA Vigna radiata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 ddNTP-sensitive DNA polymerase
-
 Vigna radiata
2.7.7.7 DNA pol
-
 Vigna radiata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.7 37
-
-
 Vigna radiata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.7 48
-
 activity is almost completely lost above 48°C Vigna radiata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.5
-
-
 Vigna radiata