EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.1.1.13 | taurocholate | increasing the bile salt concentration (0-50 mM taurocholate) in assay mixture using desalted extracts gives a correlated increase in the hydrolase activity up to 20 mM and remains more or less constant at a higher concentration of bile | Salmo salar | |
3.1.1.50 | taurocholate | 20 mM | Oncorhynchus mykiss | |
3.1.1.50 | taurocholate | 20 mM | Salmo salar |
EC Number | General Stability | Organism |
---|---|---|
3.1.1.50 | activity measurable after 4 h at 10°C | Oncorhynchus mykiss |
3.1.1.50 | activity measurable after 4 h at 10°C | Salmo salar |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.13 | Oncorhynchus mykiss | - |
- |
- |
3.1.1.13 | Salmo salar | - |
Atlantic salmon | - |
3.1.1.50 | Oncorhynchus mykiss | - |
- |
- |
3.1.1.50 | Salmo salar | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.1.13 | midgut | - |
Oncorhynchus mykiss | - |
3.1.1.13 | midgut | - |
Salmo salar | - |
3.1.1.50 | intestine | crude intestine extract, mid-gut, 10-12 h after feeding | Oncorhynchus mykiss | - |
3.1.1.50 | intestine | crude intestine extract, mid-gut, 10-12 h after feeding | Salmo salar | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.1.50 | additional information | - |
linearly increasing activity up to 24 h of incubation | Salmo salar |
3.1.1.50 | additional information | - |
linearly increasing activity with increasing protein concentration up to 200 microgram protein in the assay | Salmo salar |
3.1.1.50 | additional information | - |
linearly increasing activity with increasing substrate concentration, steep increase up to 125 microM oleic acid | Salmo salar |
3.1.1.50 | 0.000072 | - |
crude mid-gut extracts, desalted | Salmo salar |
3.1.1.50 | 0.000254 | - |
crude mid-gut extracts | Salmo salar |
3.1.1.50 | 0.000278 | - |
crude mid-gut extracts, desalted | Oncorhynchus mykiss |
3.1.1.50 | 0.000305 | - |
crude mid-gut extracts | Oncorhynchus mykiss |
3.1.1.50 | 0.000625 | - |
crude mid-gut extracts, desalted, in presence of 20 mM taurocholate | Oncorhynchus mykiss |
3.1.1.50 | 0.00063 | - |
crude mid-gut extracts, desalted, in presence of 20 mM taurocholate and trypsin inhibitor | Salmo salar |
3.1.1.50 | 0.000665 | - |
crude mid-gut extracts, desalted, in presence of 20 mM taurocholate | Salmo salar |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.1.13 | cholesteryl oleate + H2O | - |
Oncorhynchus mykiss | cholesterol + oleate | - |
? | |
3.1.1.13 | cholesteryl oleate + H2O | - |
Salmo salar | cholesterol + oleate | - |
? | |
3.1.1.13 | palmityl oleate + H2O | - |
Oncorhynchus mykiss | palmitol + oleate | - |
? | |
3.1.1.13 | palmityl oleate + H2O | - |
Salmo salar | palmitol + oleate | - |
? | |
3.1.1.13 | triolein + H2O | - |
Oncorhynchus mykiss | diolein + oleate | - |
? | |
3.1.1.13 | triolein + H2O | - |
Salmo salar | diolein + oleate | - |
? | |
3.1.1.50 | palmityl oleate + H2O | 4 h, 10°C, in presence or absence of 20 mM bile salt (taurocholate) | Oncorhynchus mykiss | hexadecanol + oleic acid | reaction stop with chloroform/methanol/toluene mixture | ? | |
3.1.1.50 | palmityl oleate + H2O | 4 h, 10°C, in presence or absence of 20 mM bile salt (taurocholate) | Salmo salar | hexadecanol + oleic acid | reaction stop with chloroform/methanol/toluene mixture | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.1.13 | bile salt-dependent lipase | contains triacylglycerol hydrolase, sterol ester hydrolase, and wax ester hydrolase activities | Oncorhynchus mykiss |
3.1.1.13 | bile salt-dependent lipase | contains triacylglycerol hydrolase, sterol ester hydrolase, and wax ester hydrolase activities | Salmo salar |
3.1.1.13 | BSDL | - |
Oncorhynchus mykiss |
3.1.1.13 | BSDL | - |
Salmo salar |
3.1.1.50 | wax ester hydrolase | - |
Oncorhynchus mykiss |
3.1.1.50 | wax ester hydrolase | - |
Salmo salar |
3.1.1.50 | WEH | - |
Oncorhynchus mykiss |
3.1.1.50 | WEH | - |
Salmo salar |