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Literature summary extracted from

  • Mizanur, R.M.; Pohl, N.L.
    Bacterial CMP-sialic acid synthetases: production, properties, and applications (2008), Appl. Microbiol. Biotechnol., 80, 757-765.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.43 additional information no enhancement of activity with dithiothreitol, glutathione, or beta-mercaptoethanol Mannheimia haemolytica

General Stability

EC Number General Stability Organism
2.7.7.43 the enzyme is equally stable with or without the presence of glycerol at different temperatures Acetivibrio thermocellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 5-mercury-CTP
-
Escherichia coli
2.7.7.43 EDTA treatment with EDTA results in loss of catalytic activity which can be restored by the addition of either Mg2+ or Mn2+ Escherichia coli
2.7.7.43 additional information dithiothreitol has little effect on enzyme activity [Haemophilus] ducreyi
2.7.7.43 p-hydroxymercuribenzoic acid
-
Mannheimia haemolytica
2.7.7.43 periodate-oxidized CTP
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.035
-
CTP at pH 7.1 and 37°C [Haemophilus] ducreyi
2.7.7.43 0.13
-
N-acylneuraminate
-
Acetivibrio thermocellus
2.7.7.43 0.24
-
CTP
-
Acetivibrio thermocellus
2.7.7.43 0.26
-
N-acylneuraminate at pH 7.1 and 37°C [Haemophilus] ducreyi
2.7.7.43 0.31
-
CTP
-
Neisseria meningitidis
2.7.7.43 0.31
-
CTP at 37°C Escherichia coli
2.7.7.43 0.34
-
N-acylneuraminate
-
Neisseria meningitidis
2.7.7.43 1.77
-
CTP
-
Mannheimia haemolytica
2.7.7.43 1.82
-
N-acylneuraminate
-
Mannheimia haemolytica
2.7.7.43 4
-
N-acylneuraminate at 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Ca2+ partially substitutes Mg2+ in catalysis Mannheimia haemolytica
2.7.7.43 Co2+ partially substitutes Mg2+ in catalysis Mannheimia haemolytica
2.7.7.43 Mg2+ required for activity Neisseria meningitidis
2.7.7.43 Mg2+ absolutely required for activity, 20 mM Mg2+ gives the most activity [Haemophilus] ducreyi
2.7.7.43 Mg2+ required for activity, 20-40 mM Mg2+ is the optimum concentration Escherichia coli
2.7.7.43 Mg2+ required for activity, maximum activity at 10 mM Mg2+, decreasing at higher Mg2+ concentrations Mannheimia haemolytica
2.7.7.43 Mn2+ required for activity Neisseria meningitidis
2.7.7.43 Mn2+ partially substitutes Mg2+ in catalysis Mannheimia haemolytica
2.7.7.43 Mn2+ required for activity, can be replaced by Mg2+, Co2+, and Zn2+ in decreasing order Acetivibrio thermocellus
2.7.7.43 Mn2+ required for activity, Mn2+ is only 40% as effective as Mg2+ at 20 mM [Haemophilus] ducreyi
2.7.7.43 additional information Ca2+ shows no effect on enzyme activity [Haemophilus] ducreyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 24800
-
gel filtration Neisseria meningitidis
2.7.7.43 25400
-
gel filtration [Haemophilus] ducreyi
2.7.7.43 26000
-
SDS-PAGE Acetivibrio thermocellus
2.7.7.43 43000
-
SDS-PAGE Mannheimia haemolytica
2.7.7.43 49000
-
SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate Escherichia coli
-
diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate Neisseria meningitidis
-
diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate Mannheimia haemolytica
-
diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate Acetivibrio thermocellus
-
diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate [Haemophilus] ducreyi
-
diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate Escherichia coli K-235
-
diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate Mannheimia haemolytica A2
-
diphosphate + CMP-N-acylneuraminate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Acetivibrio thermocellus
-
-
-
2.7.7.43 Escherichia coli
-
-
-
2.7.7.43 Escherichia coli K-235
-
-
-
2.7.7.43 Mannheimia haemolytica
-
-
-
2.7.7.43 Mannheimia haemolytica A2
-
-
-
2.7.7.43 Neisseria meningitidis
-
-
-
2.7.7.43 [Haemophilus] ducreyi
-
-
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 -20°C or -80°C, in 50% (v/v) glycerol, several months, remains stable [Haemophilus] ducreyi
2.7.7.43 4°C, at pH 7.0, several weeks, remains stable Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CDP + N-acetylneuraminate
-
Mannheimia haemolytica phosphate + CMP-N-acetylneuraminate
-
?
2.7.7.43 CDP + N-acetylneuraminate
-
Mannheimia haemolytica A2 phosphate + CMP-N-acetylneuraminate
-
?
2.7.7.43 CTP + (N-4-O-)diacetylneuraminic acid (N-4-O-)diacetylneuraminic acid is 46% effective compared to N-acylneuraminate Neisseria meningitidis diphosphate + CMP-N-4-O-diacetylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
Escherichia coli diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
Neisseria meningitidis diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
Mannheimia haemolytica diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
Acetivibrio thermocellus diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
[Haemophilus] ducreyi diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
Escherichia coli K-235 diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-acylneuraminate
-
Mannheimia haemolytica A2 diphosphate + CMP-N-acylneuraminate
-
?
2.7.7.43 CTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica CMP-N-glycolylneuraminate + diphosphate
-
?
2.7.7.43 CTP + N-glycolylneuraminic acid
-
Acetivibrio thermocellus CMP-N-glycolylneuraminate + diphosphate
-
?
2.7.7.43 CTP + N-glycolylneuraminic acid
-
[Haemophilus] ducreyi CMP-N-glycolylneuraminate + diphosphate
-
?
2.7.7.43 CTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica A2 CMP-N-glycolylneuraminate + diphosphate
-
?
2.7.7.43 additional information does not accept N-glycolylneuraminic acid and triacetylneuraminic acid as substrates Neisseria meningitidis ?
-
?
2.7.7.43 additional information does not utilize N-glycolylneuraminic acid as a substrate Escherichia coli ?
-
?
2.7.7.43 additional information does not utilize N-glycolylneuraminic acid as a substrate Escherichia coli K-235 ?
-
?
2.7.7.43 TTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica TMP-N-glycolylneuraminate + diphosphate
-
?
2.7.7.43 TTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica A2 TMP-N-glycolylneuraminate + diphosphate
-
?
2.7.7.43 UDP + N-acylneuraminate
-
Mannheimia haemolytica phosphate + UMP-N-acetylneuraminate
-
?
2.7.7.43 UDP + N-acylneuraminate
-
Mannheimia haemolytica A2 phosphate + UMP-N-acetylneuraminate
-
?
2.7.7.43 UTP + N-glycolylneuraminic acid
-
Mannheimia haemolytica UMP-N-glycolylneuraminate + diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.43 CMP-N-acetylneuraminic acid synthetase
-
Escherichia coli
2.7.7.43 CMP-N-acetylneuraminic acid synthetase
-
Neisseria meningitidis
2.7.7.43 CMP-N-acetylneuraminic acid synthetase
-
Mannheimia haemolytica
2.7.7.43 CMP-N-acetylneuraminic acid synthetase
-
Acetivibrio thermocellus
2.7.7.43 CMP-N-acetylneuraminic acid synthetase
-
[Haemophilus] ducreyi
2.7.7.43 CSS
-
Escherichia coli
2.7.7.43 CSS
-
Neisseria meningitidis
2.7.7.43 CSS
-
Mannheimia haemolytica
2.7.7.43 CSS
-
Acetivibrio thermocellus
2.7.7.43 CSS
-
[Haemophilus] ducreyi
2.7.7.43 cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Escherichia coli
2.7.7.43 cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Neisseria meningitidis
2.7.7.43 cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Mannheimia haemolytica
2.7.7.43 cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
Acetivibrio thermocellus
2.7.7.43 cytidine 5'-monophosphate N-acetylneuraminic acid synthetase
-
[Haemophilus] ducreyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 37
-
-
Escherichia coli
2.7.7.43 37
-
-
Neisseria meningitidis
2.7.7.43 37
-
-
[Haemophilus] ducreyi
2.7.7.43 50
-
-
Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.43 40 45 the enzyme loses complete activity after 50 and 10 min when stored at 40°C and 45°C, respectively Escherichia coli
2.7.7.43 70
-
the enzyme retains activity at 70°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 8 9.5
-
[Haemophilus] ducreyi
2.7.7.43 8.5
-
-
Neisseria meningitidis
2.7.7.43 9
-
-
Mannheimia haemolytica
2.7.7.43 9 10
-
Escherichia coli
2.7.7.43 9.5
-
-
Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.43 7 13
-
Acetivibrio thermocellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.43 7 12.5 activity is drastically reduced below pH 7.0 and above pH 12.5 Mannheimia haemolytica