EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | additional information | no enhancement of activity with dithiothreitol, glutathione, or beta-mercaptoethanol | Mannheimia haemolytica |
EC Number | General Stability | Organism |
---|---|---|
2.7.7.43 | the enzyme is equally stable with or without the presence of glycerol at different temperatures | Acetivibrio thermocellus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | 5-mercury-CTP | - |
Escherichia coli | |
2.7.7.43 | EDTA | treatment with EDTA results in loss of catalytic activity which can be restored by the addition of either Mg2+ or Mn2+ | Escherichia coli | |
2.7.7.43 | additional information | dithiothreitol has little effect on enzyme activity | [Haemophilus] ducreyi | |
2.7.7.43 | p-hydroxymercuribenzoic acid | - |
Mannheimia haemolytica | |
2.7.7.43 | periodate-oxidized CTP | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.43 | 0.035 | - |
CTP | at pH 7.1 and 37°C | [Haemophilus] ducreyi | |
2.7.7.43 | 0.13 | - |
N-acylneuraminate | - |
Acetivibrio thermocellus | |
2.7.7.43 | 0.24 | - |
CTP | - |
Acetivibrio thermocellus | |
2.7.7.43 | 0.26 | - |
N-acylneuraminate | at pH 7.1 and 37°C | [Haemophilus] ducreyi | |
2.7.7.43 | 0.31 | - |
CTP | - |
Neisseria meningitidis | |
2.7.7.43 | 0.31 | - |
CTP | at 37°C | Escherichia coli | |
2.7.7.43 | 0.34 | - |
N-acylneuraminate | - |
Neisseria meningitidis | |
2.7.7.43 | 1.77 | - |
CTP | - |
Mannheimia haemolytica | |
2.7.7.43 | 1.82 | - |
N-acylneuraminate | - |
Mannheimia haemolytica | |
2.7.7.43 | 4 | - |
N-acylneuraminate | at 37°C | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.43 | Ca2+ | partially substitutes Mg2+ in catalysis | Mannheimia haemolytica | |
2.7.7.43 | Co2+ | partially substitutes Mg2+ in catalysis | Mannheimia haemolytica | |
2.7.7.43 | Mg2+ | required for activity | Neisseria meningitidis | |
2.7.7.43 | Mg2+ | absolutely required for activity, 20 mM Mg2+ gives the most activity | [Haemophilus] ducreyi | |
2.7.7.43 | Mg2+ | required for activity, 20-40 mM Mg2+ is the optimum concentration | Escherichia coli | |
2.7.7.43 | Mg2+ | required for activity, maximum activity at 10 mM Mg2+, decreasing at higher Mg2+ concentrations | Mannheimia haemolytica | |
2.7.7.43 | Mn2+ | required for activity | Neisseria meningitidis | |
2.7.7.43 | Mn2+ | partially substitutes Mg2+ in catalysis | Mannheimia haemolytica | |
2.7.7.43 | Mn2+ | required for activity, can be replaced by Mg2+, Co2+, and Zn2+ in decreasing order | Acetivibrio thermocellus | |
2.7.7.43 | Mn2+ | required for activity, Mn2+ is only 40% as effective as Mg2+ at 20 mM | [Haemophilus] ducreyi | |
2.7.7.43 | additional information | Ca2+ shows no effect on enzyme activity | [Haemophilus] ducreyi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 24800 | - |
gel filtration | Neisseria meningitidis |
2.7.7.43 | 25400 | - |
gel filtration | [Haemophilus] ducreyi |
2.7.7.43 | 26000 | - |
SDS-PAGE | Acetivibrio thermocellus |
2.7.7.43 | 43000 | - |
SDS-PAGE | Mannheimia haemolytica |
2.7.7.43 | 49000 | - |
SDS-PAGE | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.43 | CTP + N-acylneuraminate | Escherichia coli | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | Neisseria meningitidis | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | Mannheimia haemolytica | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | Acetivibrio thermocellus | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | [Haemophilus] ducreyi | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | Escherichia coli K-235 | - |
diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | Mannheimia haemolytica A2 | - |
diphosphate + CMP-N-acylneuraminate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.43 | Acetivibrio thermocellus | - |
- |
- |
2.7.7.43 | Escherichia coli | - |
- |
- |
2.7.7.43 | Escherichia coli K-235 | - |
- |
- |
2.7.7.43 | Mannheimia haemolytica | - |
- |
- |
2.7.7.43 | Mannheimia haemolytica A2 | - |
- |
- |
2.7.7.43 | Neisseria meningitidis | - |
- |
- |
2.7.7.43 | [Haemophilus] ducreyi | - |
- |
- |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.43 | -20°C or -80°C, in 50% (v/v) glycerol, several months, remains stable | [Haemophilus] ducreyi |
2.7.7.43 | 4°C, at pH 7.0, several weeks, remains stable | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.43 | CDP + N-acetylneuraminate | - |
Mannheimia haemolytica | phosphate + CMP-N-acetylneuraminate | - |
? | |
2.7.7.43 | CDP + N-acetylneuraminate | - |
Mannheimia haemolytica A2 | phosphate + CMP-N-acetylneuraminate | - |
? | |
2.7.7.43 | CTP + (N-4-O-)diacetylneuraminic acid | (N-4-O-)diacetylneuraminic acid is 46% effective compared to N-acylneuraminate | Neisseria meningitidis | diphosphate + CMP-N-4-O-diacetylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Escherichia coli | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Neisseria meningitidis | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Mannheimia haemolytica | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Acetivibrio thermocellus | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
[Haemophilus] ducreyi | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Escherichia coli K-235 | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | diphosphate + CMP-N-acylneuraminate | - |
? | |
2.7.7.43 | CTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | CTP + N-glycolylneuraminic acid | - |
Acetivibrio thermocellus | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | CTP + N-glycolylneuraminic acid | - |
[Haemophilus] ducreyi | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | CTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica A2 | CMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | additional information | does not accept N-glycolylneuraminic acid and triacetylneuraminic acid as substrates | Neisseria meningitidis | ? | - |
? | |
2.7.7.43 | additional information | does not utilize N-glycolylneuraminic acid as a substrate | Escherichia coli | ? | - |
? | |
2.7.7.43 | additional information | does not utilize N-glycolylneuraminic acid as a substrate | Escherichia coli K-235 | ? | - |
? | |
2.7.7.43 | TTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica | TMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | TTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica A2 | TMP-N-glycolylneuraminate + diphosphate | - |
? | |
2.7.7.43 | UDP + N-acylneuraminate | - |
Mannheimia haemolytica | phosphate + UMP-N-acetylneuraminate | - |
? | |
2.7.7.43 | UDP + N-acylneuraminate | - |
Mannheimia haemolytica A2 | phosphate + UMP-N-acetylneuraminate | - |
? | |
2.7.7.43 | UTP + N-glycolylneuraminic acid | - |
Mannheimia haemolytica | UMP-N-glycolylneuraminate + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.43 | CMP-N-acetylneuraminic acid synthetase | - |
Escherichia coli |
2.7.7.43 | CMP-N-acetylneuraminic acid synthetase | - |
Neisseria meningitidis |
2.7.7.43 | CMP-N-acetylneuraminic acid synthetase | - |
Mannheimia haemolytica |
2.7.7.43 | CMP-N-acetylneuraminic acid synthetase | - |
Acetivibrio thermocellus |
2.7.7.43 | CMP-N-acetylneuraminic acid synthetase | - |
[Haemophilus] ducreyi |
2.7.7.43 | CSS | - |
Escherichia coli |
2.7.7.43 | CSS | - |
Neisseria meningitidis |
2.7.7.43 | CSS | - |
Mannheimia haemolytica |
2.7.7.43 | CSS | - |
Acetivibrio thermocellus |
2.7.7.43 | CSS | - |
[Haemophilus] ducreyi |
2.7.7.43 | cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Escherichia coli |
2.7.7.43 | cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Neisseria meningitidis |
2.7.7.43 | cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Mannheimia haemolytica |
2.7.7.43 | cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
Acetivibrio thermocellus |
2.7.7.43 | cytidine 5'-monophosphate N-acetylneuraminic acid synthetase | - |
[Haemophilus] ducreyi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 37 | - |
- |
Escherichia coli |
2.7.7.43 | 37 | - |
- |
Neisseria meningitidis |
2.7.7.43 | 37 | - |
- |
[Haemophilus] ducreyi |
2.7.7.43 | 50 | - |
- |
Acetivibrio thermocellus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 40 | 45 | the enzyme loses complete activity after 50 and 10 min when stored at 40°C and 45°C, respectively | Escherichia coli |
2.7.7.43 | 70 | - |
the enzyme retains activity at 70°C | Acetivibrio thermocellus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 8 | 9.5 | - |
[Haemophilus] ducreyi |
2.7.7.43 | 8.5 | - |
- |
Neisseria meningitidis |
2.7.7.43 | 9 | - |
- |
Mannheimia haemolytica |
2.7.7.43 | 9 | 10 | - |
Escherichia coli |
2.7.7.43 | 9.5 | - |
- |
Acetivibrio thermocellus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 7 | 13 | - |
Acetivibrio thermocellus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.43 | 7 | 12.5 | activity is drastically reduced below pH 7.0 and above pH 12.5 | Mannheimia haemolytica |