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Literature summary extracted from

  • Li, X.; Chi, Z.; Liu, Z.; Yan, K.; Li, H.
    Phytase production by a marine yeast Kodamea ohmeri BG3 (2008), Appl. Biochem. Biotechnol., 149, 183-193.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.3.8 synthesis the optimal medium for phytase production contains oat 10.0 g/l, ammonium sulfate 15.0 g/l, glucose 30 g/l, and NaCl 20.0 g/l, while the optimal cultivation conditions for phytase production are pH 5.0, a temperature of 28°C, and a shaking speed of 170 rpm. Under the optimal conditions, over 557.9 mU/ml of phytase activity is produced within 72 h of fermentation at the shake flask level Kodamaea ohmeri
3.1.3.26 biotechnology ten bean cultivars are evaluated for variability in phytate, phenolic, and mineral contents, phytase activity, and antioxidant properties to elucidate the relationship of these components. Multivariate data analysis performed on 22 components analyzed in this study using principal component analysis and cluster methods demonstrate that differences in phytase, antioxidant activity, mineral contents, and bioavailability are much larger within market class than among bean cultivars Kodamaea ohmeri

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.8 extracellular
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 Kodamaea ohmeri
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Kodamaea ohmeri
-
-
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3.1.3.8 Kodamaea ohmeri BG3
-
-
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3.1.3.26 Kodamaea ohmeri
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 the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 the optimal medium for phytase production contained oat 10.0 g/l, NH4SO4 15.0 g/l, glucose 30 g/l, and NaCl 20.0 g/l, while the optimal cultivation conditions for phytase production are pH 5.0, a temperature of 28°C, and a shaking speed of 170 rpm. Under the optimal conditions, over 557.9 mU/ml of phytase activity is produced within 72 h of fermentation at the shake flask level Kodamaea ohmeri

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 additional information
-
 phytic acid content and phytase activity vary significantly among cultivars and market classes, ranging from 16.7 to 25.1 g/kg and from 224 to 361 phytase activity (liberation of inorganic phosphate) micromol/min/kg of sample, respectively Kodamaea ohmeri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Kodamaea ohmeri ? + phosphate
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 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Kodamaea ohmeri BG3 ? + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 4-phytase
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 Kodamaea ohmeri

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 65
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-
 Kodamaea ohmeri
3.1.3.26 65
-
-
 Kodamaea ohmeri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 5
-
-
 Kodamaea ohmeri
3.1.3.26 5
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-
 Kodamaea ohmeri