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Literature summary extracted from

  • Yuk, J.M.; Yang, C.S.; Shin, D.M.; Kim, K.K.; Lee, S.K.; Song, Y.J.; Lee, H.M.; Cho, C.H.; Jeon, B.H.; Jo, E.K.
    A Dual Regulatory Role of Apurinic/apyrimidinic Endonuclease 1/Redox Factor-1 in HMGB1-induced Inflammatory Responses (2008), Antioxid. Redox Signal., 11, 575-587.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.99.18 lipopolysaccharide 100 ng/ml lipopolysaccharide stimulates the upregulation and nuclear translocation of APE1 in activated macrophages. APE1 critically mediates both the translocation of NF-B to the nucleus and the expression of inducible nitric oxide synthase by murine macrophage RAW264.7 cells after stimulation with LPS Homo sapiens

Application

EC Number Application Comment Organism
4.2.99.18 medicine cancer therapy, inflammatory diseases Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.99.18 wild-type APE1 encoding full-length of APE1 and APE1 C65A/C93A encoding mutant form of APE1 in pCMVTag2B mammalian expression vector are generated by standard cloning method. TAT-APE1 is generated by insertion of full-length of APE1 into pTAT-2.1 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.99.18 C65A/C93A mutant form of TAT-APE1 is generated by insertion of full-length of APE1 C65A/C93A into pTAT-2.1 Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.99.18 additional information APE1 silencing via siRNA transfection inhibits both the nuclear and cytoplasmic expression of APE1 Homo sapiens
4.2.99.18 polyinosinic-polycytidylic acid transfection of APE1 suppresses the extracellular release of high-mobility group box 1 in response to polyinosinic-polycytidylic acid stimulation Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.99.18 cytoplasm weak and diffuse cytosolic distribution under basal conditions Homo sapiens 5737
-
4.2.99.18 additional information high-mobility group box 1 stimulation induces the nuclear translocation of APE1 protein, and siRNA transfection and adenoviral transduction results in differential expression and localization patterns in activated macrophages Homo sapiens
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-
4.2.99.18 nucleus When THP-1 cells are incubated with high-mobility group box 1 for 12 h, cytoplasmic APE1 proteins are substantially translocated into nucleus. APE1 proteins are significantly translocated from cytoplasmic to nuclear fractions in THP-1 cells at 24 h after high-mobility group box 1 stimulation. Overexpression of APE1 in human primary monocytes with adenoviral transduction leads to abundant protein expression in the cytoplasmic and nuclear fractions. Homo sapiens 5634
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.99.18 37000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.99.18 additional information Homo sapiens forced cytoplasmic overexpression of APE1 profoundly attenuates the upregulation of high-mobility group box 1-mediated reactive oxygen species generation, cytokine secretion, and cyclooxygenase-2 expression by primary monocytes and macrophage-like THP-1 cell lines ?
-
 ?
4.2.99.18 additional information Homo sapiens high-mobility group box 1-induced activation of p38 and c-Jun N-terminal kinase is strongly abrogated by the overexpression of APE1 ?
-
 ?
4.2.99.18 additional information Homo sapiens multifunctional protein involved in reduction-oxidation regulation. It functions as a redox factor that maintains transcription factors in an active reduced state ?
-
 ?
4.2.99.18 additional information Homo sapiens small interfering RNA knockdown of endogenous APE1 impairs high-mobility group box 1-mediated cytokine expression and MAPK activation in THP-1 cells. High-mobility group box 1-stimulation induces the translocation of APE1 to the nucleus of the cell ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.99.18 Homo sapiens
-
-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.99.18 monocyte prepared from peripheral blood mononuclear cells Homo sapiens
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4.2.99.18 THP-1 cell human acute monocytic leukemia cell line Homo sapiens
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.99.18 additional information forced cytoplasmic overexpression of APE1 profoundly attenuates the upregulation of high-mobility group box 1-mediated reactive oxygen species generation, cytokine secretion, and cyclooxygenase-2 expression by primary monocytes and macrophage-like THP-1 cell lines Homo sapiens ?
-
 ?
4.2.99.18 additional information high-mobility group box 1-induced activation of p38 and c-Jun N-terminal kinase is strongly abrogated by the overexpression of APE1 Homo sapiens ?
-
 ?
4.2.99.18 additional information multifunctional protein involved in reduction-oxidation regulation. It functions as a redox factor that maintains transcription factors in an active reduced state Homo sapiens ?
-
 ?
4.2.99.18 additional information small interfering RNA knockdown of endogenous APE1 impairs high-mobility group box 1-mediated cytokine expression and MAPK activation in THP-1 cells. High-mobility group box 1-stimulation induces the translocation of APE1 to the nucleus of the cell Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.99.18 APE1
-
 Homo sapiens
4.2.99.18 apurinic/apyrimidinic endonuclease 1/redox factor-1
-
 Homo sapiens