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Literature summary extracted from
- An Escherichia coli expression system for glutamyl endopeptidases optimized by complete suppression of autodegradation (2008), Anal. Biochem., 381, 74-80.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.19 | Thermolysin | - |
Staphylococcus aureus | |
| 3.4.21.19 | Thermolysin | - |
Staphylococcus epidermidis | |
| 3.4.21.19 | Thermolysin | - |
Staphylococcus warneri |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.19 | expressed in Escherichia coli | Staphylococcus aureus |
| 3.4.21.19 | expressed in Escherichia coli | Staphylococcus epidermidis |
| 3.4.21.19 | expressed in Escherichia coli | Staphylococcus warneri |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.21.19 | additional information | GluV8mut5-SW, prepro-GluV8mut5 attached to mature GluSW | Staphylococcus warneri |
| 3.4.21.19 | additional information | GluV8mut5, full-length GluV8 with five substitutions (Asp36His/Glu62Gln/Glu65Ser/Ala67Pro/Asn68Ser) in the prosegment, GluV8mut5DELTAC, GluV8mut5 with C-terminal 52 residues deleted, GluV8mut4nCSer237Ala, GluV8mut4nC with an amino acid substitution (Ser237Ala), GluV8mut5-SW, prepro-GluV8mut5 attached to mature GluSW | Staphylococcus aureus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.19 | 35000 | - |
SDS-PAGE, wild-type GluSW | Staphylococcus warneri |
| 3.4.21.19 | 38000 | - |
GluV8mut5-SW, SDS-PAGE | Staphylococcus aureus |
| 3.4.21.19 | 38000 | - |
SDS-PAGE , GluV8mut5-SW | Staphylococcus warneri |
| 3.4.21.19 | 44000 | - |
SDS-PAGE. GluV8 is scarcely expressed. Mutant GluV8mut5 shows a 44 kDa band having much higher density than that of GluV8mut4 | Staphylococcus aureus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.19 | Staphylococcus aureus | - |
- |
- |
| 3.4.21.19 | Staphylococcus aureus V8 | - |
- |
- |
| 3.4.21.19 | Staphylococcus epidermidis | - |
- |
- |
| 3.4.21.19 | Staphylococcus warneri | - |
JCM 2415 | - |
| 3.4.21.19 | Staphylococcus warneri JCM 2415 | - |
JCM 2415 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.19 | - |
Staphylococcus epidermidis |
| 3.4.21.19 | - |
Staphylococcus warneri |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.19 | Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O | the C-terminal tripeptide of the prosequence of GluV8 (i.e., His66-Ala-Asn68) resembles the unprocessed GluV8 cleavable substrate Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide | Staphylococcus aureus | ? | - |
? |  |
| 3.4.21.19 | Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O | the C-terminal tripeptide of the prosequence of GluV8 (i.e., His66-Ala-Asn68) resembles the unprocessed GluV8 cleavable substrate Ac-Ala-Ala-Asn-4-methylcoumaryl-7-amide | Staphylococcus aureus V8 | ? | - |
? | |
| 3.4.21.19 | Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O | GluV8 also possesses trace activity toward Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide that is at least 30fold higher than the activity for residual 4-methylcoumaryl-7-amide peptides that carried Ala, Phe, or Leu at their P1 position | Staphylococcus aureus | ? | - |
? | |
| 3.4.21.19 | Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide + H2O | GluV8 also possesses trace activity toward Z-Ala-Ala-Asn-4-methylcoumaryl-7-amide that is at least 30fold higher than the activity for residual 4-methylcoumaryl-7-amide peptides that carried Ala, Phe, or Leu at their P1 position | Staphylococcus aureus V8 | ? | - |
? | |
| 3.4.21.19 | Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O | - |
Staphylococcus aureus | ? | - |
? | |
| 3.4.21.19 | Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O | - |
Staphylococcus epidermidis | ? | - |
? | |
| 3.4.21.19 | Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O | - |
Staphylococcus warneri | ? | - |
? | |
| 3.4.21.19 | Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O | - |
Staphylococcus warneri JCM 2415 | ? | - |
? | |
| 3.4.21.19 | Z-Leu-Leu-Glu-4-methylcoumaryl-7-amide + H2O | - |
Staphylococcus aureus V8 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.19 | GluSE | - |
Staphylococcus epidermidis |
| 3.4.21.19 | GluSW | - |
Staphylococcus warneri |
| 3.4.21.19 | glutamyl endopeptidase | - |
Staphylococcus epidermidis |
| 3.4.21.19 | glutamyl endopeptidase | - |
Staphylococcus warneri |
| 3.4.21.19 | glutamyl endopeptidase I | - |
Staphylococcus aureus |
| 3.4.21.19 | GluV8 | - |
Staphylococcus aureus |
| 3.4.21.19 | V8 protease | - |
Staphylococcus aureus |
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Release 2023.1 (January 2023)
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