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Literature summary extracted from

  • Ngo, P.H.; Natarajan, S.; Kim, H.; Hung, H.K.; Kim, J.; Lee, B.; Ahn, Y.; Kang, L.
    Cloning, expression, crystallization and preliminary x-ray crystallographic analysis of 3-dehydroquinate synthase, Xoo1243, from Xanthomonas oryzae pv. oryzae (2008), Acta Crystallogr. Sect. F, 64, 1128-1131.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.3.4 drug development potential antibiotic target Xanthomonas oryzae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.4 The aroB gene cloned from Xoo and the corresponding DHQS protein is subsequently overexpressed in Escherichia coli Xanthomonas oryzae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.3.4 by using the hanging-drop vapour-diffusion method with different screening kits, yields crystals belonging to the tetragonal space group, crystals of DHQS obtained in sitting-drop setups using a hydra automated high-throughput crystal screening machine with different screening kits: (a) 0.1 M sodium malonate pH 4.0, 12% PEG 3350, (b) 0.2 M sodium malonate pH 6.0, 20% PEG 3350, (c) 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate, (d) 0.15 M caesium chloride, 15% PEG 3350, (e) 1.0 M sodium citrate, 0.1 M CHES pH 9.5, (f) 0.2 M ammonium sulfate, 0.1 M bis-Tris pH 5.5, 25% PEG 3350, (g) 0.2 M ammonium sulfate, 0.1 M MES pH 6.5, 30% PEG MME 5000, (h) 0.02 M zinc chloride, 20% PEG 3350. Best crystals are obtained after two weeks from a reservoir containing 0.2 M ammonium sulfate, 0.1 M MES monohydrate pH 6.0 and 31%PEG MME 5000 Xanthomonas oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.4 3-deoxy-D-arabinoheptulosonate 7-phosphate Xanthomonas oryzae shikimate pathway is present in all organisms except mammals 3-dehydroquinate + phosphate
-
 ?
4.2.3.4 additional information Xanthomonas oryzae enzyme plays an essential role in the synthesis of aromatic compounds in the shikimate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.4 Xanthomonas oryzae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.4 by Ni2+-NTA affinity chromatography, enzyme is subjected to a TEV protease cleavage reaction to cleave the six His-tag residues at the N-terminus, further purification by ion-exchange chromatography Xanthomonas oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.4 additional information
-
 Inactivation of enzymes in the shikimate pathway results in attenuation of virulence and loss of viability in a large number of bacterial species Xanthomonas oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.4 3-deoxy-D-arabinoheptulosonate 7-phosphate shikimate pathway is present in all organisms except mammals Xanthomonas oryzae 3-dehydroquinate + phosphate
-
 ?
4.2.3.4 additional information enzyme plays an essential role in the synthesis of aromatic compounds in the shikimate pathway Xanthomonas oryzae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.4 3-dehydroquinate synthase
-
 Xanthomonas oryzae
4.2.3.4 DHQS
-
 Xanthomonas oryzae
4.2.3.4 Xoo1243
-
 Xanthomonas oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.4 NAD+
-
 Xanthomonas oryzae