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Literature summary extracted from

  • Carrasco-Lopez, C.; Godoy, C.; de las Rivas, B.; Fernandez-Lorente, G.; Palomo, J.M.; Guisan, J.M.; Fernandez-Lafuente, R.; Martinez-Ripoll, M.; Hermoso, J.A.
    Crystallization and preliminary X-ray diffraction studies of the BTL2 lipase from the extremophilic microorganism Bacillus thermocatenulatus (2008), Acta Crystallogr. Sect. F, 64, 1043-1045.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expressed in Escherichia coli strain BL21 (DE3) Geobacillus thermocatenulatus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.3 sitting drop vapour diffusion method, mixture of 13% 2-methyl-2,4-pentanediol and 0.2 M ammonium acetate in 0.05 M sodium citrate pH 5.5-5.6, at 18°C Geobacillus thermocatenulatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 43000
-
 SDS-PAGE Geobacillus thermocatenulatus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Geobacillus thermocatenulatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 octyl-Sepharose column chromatography, Q-Sepharose column chromatography, and SP-550 Toyopearl column chromatography Geobacillus thermocatenulatus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 BTL2
-
 Geobacillus thermocatenulatus
3.1.1.3 lipase 2
-
 Geobacillus thermocatenulatus
3.1.1.3 triacylglycerol acylhydrolase
-
 Geobacillus thermocatenulatus