EC Number | Crystallization (Comment) | Organism |
---|---|---|
5.3.1.1 | mutant A178L, unliganded and in complex with 2-phosphoglycolate, to 2.2 A and 1.89 A, respectively. Monomeric mutant mlA178L, unliganded and in complex with 2-phosphoglycolate, to 2.3 A and 1.18 A, respectively. Mutation A178L favors the closed conformation of the C-terminal hinge region | Trypanosoma brucei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.3.1.1 | A178L | decrease in catalytic efficiency. Crystallization data reveal a more disordered loop-6 in the structure of unliganded A178L. Liganded structures show minimal differences to wild-type | Trypanosoma brucei |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.3.1.1 | 2-Phosphoglycolate | - |
Trypanosoma brucei | |
5.3.1.1 | AsO43- | - |
Trypanosoma brucei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.1 | 0.26 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 0.61 | - |
D-glyceraldehyde 3-phosphate | mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 0.9 | - |
dihydroxyacetone phosphate | wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 1.4 | - |
dihydroxyacetone phosphate | mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 3.3 | - |
D-glyceraldehyde 3-phosphate | monomeric mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 3.8 | - |
D-glyceraldehyde 3-phosphate | monomeric wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 7.6 | - |
dihydroxyacetone phosphate | monomeric wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 9.3 | - |
dihydroxyacetone phosphate | monomeric mutant A178L, pH 7.6, 25°C | Trypanosoma brucei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.1.1 | Trypanosoma brucei | P04789 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.1.1 | D-glyceraldehyde 3-phosphate | - |
Trypanosoma brucei | dihydroxyacetone phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.1 | 0.06 | - |
dihydroxyacetone phosphate | monomeric mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 0.25 | - |
D-glyceraldehyde 3-phosphate | monomeric mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 0.8 | - |
dihydroxyacetone phosphate | monomeric wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 2.2 | - |
D-glyceraldehyde 3-phosphate | monomeric wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 500 | - |
dihydroxyacetone phosphate | mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 800 | - |
dihydroxyacetone phosphate | wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 4400 | - |
D-glyceraldehyde 3-phosphate | wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 5300 | - |
D-glyceraldehyde 3-phosphate | mutant A178L, pH 7.6, 25°C | Trypanosoma brucei |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.3.1.1 | 0.05 | - |
2-Phosphoglycolate | wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 0.17 | - |
2-Phosphoglycolate | monomeric mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 0.39 | - |
2-Phosphoglycolate | mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 4.9 | - |
AsO43- | wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 5.1 | - |
AsO43- | monomeric wild-type, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 7.8 | - |
AsO43- | mutant A178L, pH 7.6, 25°C | Trypanosoma brucei | |
5.3.1.1 | 17.9 | - |
AsO43- | monomeric mutant A178L, pH 7.6, 25°C | Trypanosoma brucei |