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Literature summary extracted from

  • Potocka, A.; Zimowski, J.
    Metabolism of conjugated sterols in eggplant. Part 1. UDP-glucose:sterol glucosyltransferase (2008), Acta Biochim. Pol., 55, 127-134.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.173 2-mercaptoethanol
-
 Solanum melongena
2.4.1.173 additional information stimulated by acylglycerols containing unsaturated fatty acid residues Solanum melongena
2.4.1.173 Triton X-100 130fold at 0.1% Solanum melongena

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.173 2-Hydroxy-5-nitrobenzyl bromide
-
 Solanum melongena
2.4.1.173 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid
-
 Solanum melongena
2.4.1.173 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid
-
 Solanum melongena
2.4.1.173 cholesteryl glucoside
-
 Solanum melongena
2.4.1.173 Co2+
-
 Solanum melongena
2.4.1.173 diacylglycerol with saturated fatty acids Solanum melongena
2.4.1.173 diethyldicarbonate protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose Solanum melongena
2.4.1.173 monoacylglycerol with saturated fatty acids Solanum melongena
2.4.1.173 additional information inhibited at high ionic strength, 50% inhibition at 0.52 M NaF or 0.29 M NaCl; inhibited by dicarboxylic amino acids-modifying reagent Woodward’s Reagent K protected against inactivation upon pre-incubation with UDP-glucose but not with stigmast-5-en-3beta-ol; no effect with Mg2+, Ca2+, Mn2+, Tween20, Tween 40, Tween 60, Tween 80, Tyloxapol, sodium deoxycholate, EDTA, EGTA, and reagents modifying serine, arginine and tyrosine side chains Solanum melongena
2.4.1.173 N-ethylmaleimide protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose Solanum melongena
2.4.1.173 n-octyl thioglucoside irreversible inhibited, 50% of activity at 4.8 mM Solanum melongena
2.4.1.173 p-chloromercuribenzenesulfonic acid
-
 Solanum melongena
2.4.1.173 pyridoxal 5'-phosphate
-
 Solanum melongena
2.4.1.173 triacylglycerol with saturated fatty acids Solanum melongena
2.4.1.173 UDP
-
 Solanum melongena
2.4.1.173 UTP
-
 Solanum melongena
2.4.1.173 Zn2+
-
 Solanum melongena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.173 0.00143
-
 stigmast-5-en-3beta-ol pH 7,3, 30°C Solanum melongena
2.4.1.173 0.01429
-
 UDP-glucose pH 7.3, 30°C Solanum melongena

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.173 membrane membrane-bound Solanum melongena 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.173 additional information Solanum melongena may participate in the regulating of free sterol concentration in the cell ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.173 Solanum melongena
-
 cv Black Beauty, eggplant
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.173 partial Solanum melongena

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.173 leaf
-
 Solanum melongena
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.173 (22S,25S)-5alpha-solanidanin-3beta-ol + UDP-glucose demissidine 1.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 (22S,25S)-epoxy-furost-5-en-3beta,26-diol + UDP-glucose nuatigenin, 16.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 (25R)-5alpha-spirostan-3beta-ol + UDP-glucose tigogenin, 2.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 (25R)-5alpha-spirostan-3beta-ol-12-one + UDP-glucose hecogenin, 6.3% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 (25R)-spirost-5-en-3beta,25beta-diol + UDP-glucose isonuatigenin, 13.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 (25R)-spirost-5-en-3beta-ol + UDP-glucose diosgenin, 3.5% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 (25S)-5alpha,22betaN-spirosolanin-3beta-ol + UDP-glucose tomatidine, 2.8% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 5alpha-cholest-7-en-3beta-ol + UDP-glucose 14.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 5alpha-cholestan-3beta-ol + UDP-glucose cholestanol, 12.0% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 androst-5-en-3beta-ol-17-one + UDP-glucose androstenolon, 16.0% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 cholest-5-en-22-on-3beta-ol + UDP-glucose 22-oxycholesterol, 133.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 cholest-5-en-3beta,19-diol + UDP-glucose 9.2% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 cholest-5-en-3beta,20alpha-diol + UDP-glucose 90.8% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 cholest-5-en-3beta,25-diol + UDP-glucose 25-hydroxycholesterol, 89.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 cholest-5-en-3beta-ol + UDP-glucose cholesterol, 98.5% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 cholest-5-en-3beta-ol-7-one + UDP-glucose 5.6% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 additional information may participate in the regulating of free sterol concentration in the cell Solanum melongena ?
-
 ?
2.4.1.173 additional information UDP-galactose, TDP-glucose and CDP-glucose are also suitable as a sugar source for sterol glucosylation but with distinctly lower rates Solanum melongena ?
-
 ?
2.4.1.173 additional information no activity with solasodine (25R-22alphaN-spirosol-5-enin-3beta-ol), solanidine (22S,25S-solanid-5-enin-3beta-ol), sarsasapogenin (25S-5beta-spirostan-3beta-ol), yamogenin (25S-spirost-5-en-3beta-ol), thiocholesterol (cholest-5-en-3beta-tiol), 5beta-cholestan-3beta-ol (coprostanol), 5alpha-cholestan-3alpha-ol (epicholestanol), 5beta-cholestan-3alpha-ol (epicoprostanol), lanosterol (4,4’,14-trimethyl-5alpha-cholest-8-en-3beta-ol), and beta-amyrin (olean-12-en-3beta-ol) do not serve as glucose acceptors Solanum melongena ?
-
 ?
2.4.1.173 pregn-5-en-3beta-ol-20-one + UDP-glucose pregnenolon, 33.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 stigmast-5-en-3beta-ol + UDP-glucose sitosterol, 100% of the activity Solanum melongena additional information 3-O-beta-D-monoglucopyranoside of sitosterol and 3-O-beta-D-monogalactopyranoside of sitosterol identified as products ?
2.4.1.173 stigmasta-5,22-dien-3beta-ol + UDP-glucose stigmasterol, 49.2% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 stigmasta-5,24(28)-dien-3beta-ol + UDP-glucose 82.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?
2.4.1.173 stigmastan-3beta-ol + UDP-glucose 38.7% of the activity with sitosterol (stigmast-5-en-3beta-ol) Solanum melongena ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.173 UDP-glucose:sterol glucosyltransferase
-
 Solanum melongena

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.4.1.173 0.035
-
 pH 7.3, 30°C Solanum melongena UTP
2.4.1.173 0.065
-
 pH 7.3, 30°C Solanum melongena UDP
2.4.1.173 0.09
-
 pH 7.3, 30°C Solanum melongena Zn2+
2.4.1.173 0.53
-
 pH 7.3, 30°C Solanum melongena Co2+