Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Itoh, K.; Yamamoto, K.; Adachi, M.; Kosaka, T.; Tanaka, Y.
    Leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin DELTA13-reductase (LTB4 12-HD/PGR) responsible for the reduction of a double-bond of the alpha,beta-unsaturated ketone of an aryl propionic acid non-steroidal anti-inflammatory agent CS-670 (2008), Xenobiotica, 38, 249-263.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.3.1.74 ammonium sulfate stimulates activity Rattus norvegicus
1.3.1.74 CH3COO- NH4+ stimulates activity Rattus norvegicus
1.3.1.74 CH3COONa stimulates activity Rattus norvegicus
1.3.1.74 Na2S2O5 stimulates activity Rattus norvegicus
1.3.1.74 Na2SO4 stimulates activity Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.48 expressed in Escherichia coli Top10 cells Rattus norvegicus
1.3.1.74 expressed in Escherichia coli Top10 cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.48 15-keto prostaglandin F2alpha 43.06% residual activity at 0.1 mM Rattus norvegicus
1.3.1.48 benzylidene acetophenone 21.09% residual activity at 0.5 mM Rattus norvegicus
1.3.1.48 dicoumarol 11.45% residual activity at 0.1 mM Rattus norvegicus
1.3.1.48 indomethacin 14.22% residual activity at 0.1 mM Rattus norvegicus
1.3.1.48 additional information finasteride has no inhibitory effect Rattus norvegicus
1.3.1.48 quercitrin 13.14% residual activity at 0.1 mM Rattus norvegicus
1.3.1.74 15-keto prostaglandin F2alpha 43.06% residual activity at 0.1 mM Rattus norvegicus
1.3.1.74 benzylidene acetophenone 21.09% residual activity at 0.5 mM Rattus norvegicus
1.3.1.74 dicoumarol 11.45% residual activity at 0.1 mM Rattus norvegicus
1.3.1.74 indomethacin 14.22% residual activity at 0.1 mM Rattus norvegicus
1.3.1.74 additional information finasteride has no inhibitory effect Rattus norvegicus
1.3.1.74 quercitrin 13.14% residual activity at 0.1 mM Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.48 0.0078
-
 15-ketoprostaglandin E2 in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 0.0123
-
 phenyl propenyl ketone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 0.0277
-
 benzylidene acetone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 0.0791
-
 cinnamoylthiophene in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 0.1289
-
 Methyl vinyl ketone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 0.1399
-
 benzylidene acetonphenone in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 0.461
-
 4-hydroxy-2-nonenal in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 1.426
-
 cinnamaldehyde in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.48 2.194
-
 trans-2-hexenal in 100 mM phosphate buffer (pH 7.4), at 25°C Rattus norvegicus
1.3.1.74 0.02
-
 2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid at 30°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.48 cytosol
-
 Rattus norvegicus 5829
-
1.3.1.74 cytosol
-
 Rattus norvegicus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.74 Ca2+ stimulates activity Rattus norvegicus
1.3.1.74 K+ stimulates activity Rattus norvegicus
1.3.1.74 Li+ stimulates activity Rattus norvegicus
1.3.1.74 Mg2+ stimulates activity Rattus norvegicus
1.3.1.74 Mn2+ stimulates activity Rattus norvegicus
1.3.1.74 Na+ stimulates activity Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.74 35000
-
 SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.48 Rattus norvegicus
-
-
-
1.3.1.74 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.48 2',5'-ADP Sepharose 4B column chromatography and HisTrap HP column chromatography Rattus norvegicus
1.3.1.74 2',5'-ADP Sepharose 4B column chromatography, Resource Q column chromatography, and HiLoad Superdex gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.48 liver
-
 Rattus norvegicus
-
1.3.1.74 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.74 0.0054
-
 cytosol extract Rattus norvegicus
1.3.1.74 0.803
-
 after 150fold purification Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.48 11-hydroxy-9,15-dioxoprosta-5,13-dien-1-oic acid + NADPH
-
 Rattus norvegicus 11-hydroxy-9,15-dioxoprosta-5-en-1-oic acid + NADP+
-
 ?
1.3.1.48 15-ketoprostaglandin E2 + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 4-hydroxy-2-nonenal + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 benzylidene acetone + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 benzylidene acetonphenone + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 cinnamaldehyde + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 cinnamoylthiophene + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 leukotriene B4 + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 methyl vinyl ketone + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 phenyl propenyl ketone + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.48 trans-2-hexenal + NADPH
-
 Rattus norvegicus ?
-
 ?
1.3.1.74 2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid + NADPH 2-[4-[(E)-(2-oxocyclohexylidene)methyl]phenyl]propanoic acid is also known as CS-670 Rattus norvegicus 2-[4-[(2-oxocyclohexyl)methyl]phenyl]propanoic acid + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.48 15-ketoprostaglandinDELTA13-reductase
-
 Rattus norvegicus
1.3.1.48 bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase Rattus norvegicus
1.3.1.48 PGR
-
 Rattus norvegicus
1.3.1.74 bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase a beta-NADPH-dependent alkenal/one oxidoreductase which has a role for leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandinDELTA13-reductase Rattus norvegicus
1.3.1.74 CS-670 double-bond reductase
-
 Rattus norvegicus
1.3.1.74 LTB4 12-HD/PGR
-
 Rattus norvegicus
1.3.1.74 NADPH-dependent alkenal/one oxidoreductase
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.48 NADPH
-
 Rattus norvegicus
1.3.1.74 NADPH dependent Rattus norvegicus