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Literature summary extracted from

  • Ge, J.; Wei, Z.; Huang, Y.; Yin, J.; Zhou, Z.; Zhong, J.
    AcMNPV ORF38 protein has the activity of ADP-ribose pyrophosphatase and is important for virus replication (2007), Virology, 361, 204-211.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.13 expression of ORF38 as His6-tagged protein in Escherichia coli strain Bl21(DE3) Autographa californica multiple nucleopolyhedrovirus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.13 0.204
-
 ADP-ribose pH 8.0 Autographa californica multiple nucleopolyhedrovirus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.13 Mg2+ absolutely required, activates at 5 mM Autographa californica multiple nucleopolyhedrovirus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.13 ADP-ribose + H2O Autographa californica multiple nucleopolyhedrovirus
-
 AMP + D-ribose 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.13 Autographa californica multiple nucleopolyhedrovirus
-
 AcMNPV, ORF38
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.13 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Autographa californica multiple nucleopolyhedrovirus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.13 ADP-ribose + H2O
-
 Autographa californica multiple nucleopolyhedrovirus AMP + D-ribose 5-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.13 ADP-ribose pyrophosphatase
-
 Autographa californica multiple nucleopolyhedrovirus
3.6.1.13 ADPRase
-
 Autographa californica multiple nucleopolyhedrovirus
3.6.1.13 More the enzyme belongs to the Nudix, nucleotide diphosphate X,pyrophosphatase superfamily Autographa californica multiple nucleopolyhedrovirus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.13 6.96
-
 ADP-ribose pH 8.0 Autographa californica multiple nucleopolyhedrovirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.13 8
-
 assay at Autographa californica multiple nucleopolyhedrovirus