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Literature summary extracted from

  • McCoy, J.G.; Bailey, L.J.; Ng, Y.H.; Bingman, C.A.; Wrobel, R.; Weber, A.P.; Fox, B.G.; Phillips, G.N.
    Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria (2008), Proteins, 74, 368-367.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.157 expression in Escherichia coli Galdieria sulphuraria

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.157 hanging drop vapor-diffusion method, crystal structure of the enzyme, solved to a resolution of 1.95 A Galdieria sulphuraria

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.157 0.144
-
 S-adenosyl-L-methionine cosubstrate: sarcosine Galdieria sulphuraria
2.1.1.157 0.15
-
 S-adenosyl-L-methionine cosubstrate: N,N-dimethylglycine Galdieria sulphuraria
2.1.1.157 2
-
 sarcosine
-
 Galdieria sulphuraria
2.1.1.157 2.8
-
 N,N-dimethylglycine
-
 Galdieria sulphuraria

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.157 34000
-
 2 * 34000, denaturing PAGE Galdieria sulphuraria
2.1.1.157 67700
-
 gel filtration Galdieria sulphuraria

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.157 Galdieria sulphuraria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.157
-
 Galdieria sulphuraria

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.157 S-adenosyl-L-methionine + N,N-dimethylglycine
-
 Galdieria sulphuraria S-adenosyl-L-homocysteine + betaine
-
 ?
2.1.1.157 S-adenosyl-L-methionine + sarcosine
-
 Galdieria sulphuraria S-adenosyl-L-homocysteine + N,N-dimethylglycine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.157 dimer 2 * 34000, denaturing PAGE Galdieria sulphuraria

Synonyms

EC Number Synonyms Comment Organism
2.1.1.157 dimethylglycine methyltransferase
-
 Galdieria sulphuraria
2.1.1.157 GsSDMT
-
 Galdieria sulphuraria

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.157 61
-
 melting temperature Galdieria sulphuraria

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.157 1.07
-
 sarcosine
-
 Galdieria sulphuraria
2.1.1.157 1.4
-
 N,N-dimethylglycine
-
 Galdieria sulphuraria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.157 7.5
-
 methylation of dimethylglycine Galdieria sulphuraria
2.1.1.157 8
-
 methylation of sarcosine Galdieria sulphuraria