EC Number | Application | Comment | Organism |
---|---|---|---|
3.6.1.23 | drug development | the enzyme is a target for development of antimicrobial agents | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.23 | purified recombinant enzyme in complex with inhibitors, i.e. dUTPase-alpha,beta-methylene-dUDP and dUTPase-dUDP-Mn complexes, 20°C, hanging-drop vapor diffusion, 3 mg/ml of enzyme in 10 mM Tris/HCl buffer, pH 7.0, and 50 mM NaCl, as well as 1.25 mM alpha,beta-methylene-dUDP or dUDP and 10 mM MgCl2 or MnCl2, respectively, mixing with an equal volume of reservoir solution 0.1 M Tris/HCl buffer, pH 7.8, containing 18-20% PEG 3350, and 400 mM Na-acetate, X-ray diffraction structure determination and analysis at 1.7-1.84 resolution, analysis of the different alpha-phosphate sites, overview | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | 3'-deoxy-5'-O-(hydroxy{[hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl)uridine | competitive inhibition | Escherichia coli | |
3.6.1.23 | 3'-deoxy-5'-O-[hydroxy(phosphonoamino)phosphoryl]uridine | competitive inhibition | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | additional information | - |
additional information | ligand binding constants | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | - |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Escherichia coli | a preventive DNA repair enzyme, contributes to maintain the appropriate cellular dUTP/dTTP ratio by catalyzing dUTP hydrolysis, dUTPase is essential for viability | dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Escherichia coli | P06968 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | a preventive DNA repair enzyme, contributes to maintain the appropriate cellular dUTP/dTTP ratio by catalyzing dUTP hydrolysis, dUTPase is essential for viability | Escherichia coli | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | substrate binding structure, the strictly conserved residue Ser72 is involved in phosphate chain binding, overview | Escherichia coli | dUMP + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | dUTP pyrophosphatase | - |
Escherichia coli |
3.6.1.23 | dUTPase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 25 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 7.5 | - |
assay at | Escherichia coli |