Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rajaram, V.; Ratna Prasuna, P.; Savithri, H.S.; Murthy, M.R.
    Structure of biosynthetic N-acetylornithine aminotransferase from Salmonella typhimurium: studies on substrate specificity and inhibitor binding (2008), Proteins, 70, 429-441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.11 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.1.11 in unliganded form and in complex with gabaculine or L-gutamate, diffration to 1.9 A resolution. The structure of unliganded enzyme shows significant electron density for pyridoxal 5'-phosphate in subunits A which can be attributed to the ordering of the loop Lak-bm in subunit B Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.11 gabaculine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.6.1.11 NaCl 200 mM, 25% residual activity Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.11 0.037
-
 N2-Acetyl-L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium
2.6.1.11 0.64
-
 L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.11 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.11 L-ornithine + 2-oxoglutarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-glutamate-gamma-semialdehyde + L-glutamate
-
 ?
2.6.1.11 N2-acetyl-L-ornithine + 2-oxoglutarate
-
 Salmonella enterica subsp. enterica serovar Typhimurium N-acetyl-L-glutamate-gamma-semialdehyde + L-glutamate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.11 0.82
-
 L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium
2.6.1.11 1.55
-
 N2-Acetyl-L-ornithine 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.11 9.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.11 8
-
 about 50% of maximal activity Salmonella enterica subsp. enterica serovar Typhimurium

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.6.1.11 0.007
-
 25°C, pH 9.5 Salmonella enterica subsp. enterica serovar Typhimurium gabaculine