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Literature summary extracted from

  • Martinez-Rodriguez, S.; Andujar-Sanchez, M.; Neira, J.L.; Clemente-Jimenez, J.M.; Jara-Perez, V.; Rodriguez-Vico, F.; Las Heras-Vazquez, F.J.
    Site-directed mutagenesis indicates an important role of cysteines 76 and 181 in the catalysis of hydantoin racemase from Sinorhizobium meliloti (2006), Protein Sci., 15, 2729-2738.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.1.99.5 C181A no residual activity. The secondary and the tertiary structure of the mutant is not significantly affected. Residue Cys181 is responsible for L-isomer recognition and racemization. Binding thermodynamic parameters of different substrates, thermodynamic parameters of the guanidinium hydrochloride-induced denaturation Sinorhizobium meliloti
5.1.99.5 C76A no residual activity. The secondary and the tertiary structure of the mutant is not significantly affected. Residue Cys76 is responsible for recognition and proton retrieval of D-isomers. Binding thermodynamic parameters of different substrates, thermodynamic parameters of the guanidinium hydrochloride-induced denaturation Sinorhizobium meliloti

Organism

EC Number Organism UniProt Comment Textmining
5.1.99.5 Sinorhizobium meliloti
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
5.1.99.5 D-5-monosubstituted hydantoin = L-5-monosubstituted hydantoin two-base mechanism for the racemization of 5-monosubstituted hydantoins Sinorhizobium meliloti
a

Renatured (Commentary)

EC Number Renatured (Comment) Organism
5.1.99.5 thermodynamic parameters of the guanidinium hydrochloride-induced denaturation of wild-type and mutants C76A, C181A Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.99.5 L-5-methylthioethylhydantoin
-
 Sinorhizobium meliloti D-5-methylthioethylhydantoin
-
 r
5.1.99.5 L-ethylhydantoin
-
 Sinorhizobium meliloti D-ethylhydantoin
-
 r
5.1.99.5 L-isopropylhydantoin
-
 Sinorhizobium meliloti D-isopropylhydantoin
-
 r