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Literature summary extracted from

  • Goren, M.A.; Fox, B.G.
    Wheat germ cell-free translation, purification, and assembly of a functional human stearoyl-CoA desaturase complex (2008), Protein Expr. Purif., 62, 171-178.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.1 in vitro translation using a wheat germ cell-free extract in the presence of unilamelar liposomes, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.1 additional information near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, overview Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.19.1 Fe2+ heme and non-heme iron Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+ Homo sapiens
-
oleoyl-CoA + ferricytochrome b5 + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.1 Homo sapiens O00767
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.1 near complete transfer of the in vitro expressed enzyme integral membrane protein into the liposomes, followed by single step purification via centrifugation in a density gradient yielded proteoliposomes with the desaturase in high purity as judged by capillary electrophoresis, method optimization of cell-free translation to the assembly and purification of integral membrane protein complexes, overview Homo sapiens

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.19.1 in vitro reconstitution of the non-heme iron and heme active sites to a functional reconstituted enzyme complex after in vitro translation and protein integration into liposomes, method optimization, overview Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.1 stearoyl-CoA + ferrocytochrome b5 + O2 + H+
-
Homo sapiens oleoyl-CoA + ferricytochrome b5 + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.1 SCD
-
Homo sapiens
1.14.19.1 stearoyl-CoA desaturase
-
Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.1 37
-
assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.1 7.2
-
assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.1 ferrocytochrome b5
-
Homo sapiens