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Literature summary extracted from

  • Wang, J.; Tan, H.; Zhao, Z.K.
    Over-expression, purification, and characterization of recombinant NAD-malic enzyme from Escherichia coli K12 (2007), Protein Expr. Purif., 53, 97-103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.38 expression of His6-tagged enzyme in strain BL21(DE3) as mainly soluble protein Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.38 0.097
-
 NAD+ pH 7.2, 25°C, recombinant enzyme Escherichia coli K-12
1.1.1.38 0.42
-
 (S)-malate pH 7.2, 25°C, recombinant enzyme Escherichia coli K-12

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.38 Mn2+ required Escherichia coli K-12

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.38 65000
-
 monomeric recombinant His6-tagged enzyme, native PAGE Escherichia coli K-12
1.1.1.38 260000
-
 tetrameric recombinant His6-tagged enzyme, native PAGE Escherichia coli K-12
1.1.1.38 500000
-
 octameric recombinant His6-tagged enzyme, native PAGE Escherichia coli K-12

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.38 (S)-malate + NAD+ Escherichia coli K-12 via decarboxylation of oxaloacetate pyruvate + CO2 + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.38 Escherichia coli K-12
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.38 recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography and ultrafiltration to 95% purity Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.38 (S)-malate + NAD+ via decarboxylation of oxaloacetate Escherichia coli K-12 pyruvate + CO2 + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.38 oligomer x * 65000, recombinant His6-tagged enzyme, SDS-PAGE, monomer, homotetramer, and homooctamer enzyme fomrs exist in solution Escherichia coli K-12

Synonyms

EC Number Synonyms Comment Organism
1.1.1.38 NAD+-dependent malic enzyme
-
 Escherichia coli K-12
1.1.1.38 NAD-malic enzyme
-
 Escherichia coli K-12
1.1.1.38 NAD-ME
-
 Escherichia coli K-12

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.38 25
-
 assay at Escherichia coli K-12

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.38 134.4
-
 (S)-malate pH 7.2, 25°C, recombinant enzyme Escherichia coli K-12

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.38 7.2
-
 recombinant His6-tagged enzyme Escherichia coli K-12

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.38 6.9 8 over 70% of maximal activity within this range Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.38 NAD+ dependent on Escherichia coli K-12