EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.3.3 | phylogenetic tree, overview | Paracoccus sp. WB1 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.3 | Ag+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
3.5.3.3 | Co2+ | 50% inhibition at 1 mM | Paracoccus sp. WB1 | |
3.5.3.3 | Cu2+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
3.5.3.3 | Hg2+ | complete inactivation at 1 mM | Paracoccus sp. WB1 | |
3.5.3.3 | additional information | NaN3, and chelating agents EDTA and 1,10-phenanthroline do not inhibit the activity at the concentration of 20 mM, no inhibition by Tween80 and SDS, and by Ca2+, Pb2+, Ba2+, Fe2+, Fe3+, Mg2+, Cr2+, Mn2+, and Li+ | Paracoccus sp. WB1 | |
3.5.3.3 | Tween20 | strong inhibition at 1 mM | Paracoccus sp. WB1 | |
3.5.3.3 | Zn2+ | 40% inhibition at 1 mM | Paracoccus sp. WB1 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.3.3 | 0.034 | - |
Creatine | pH 7.5, 37°C | Paracoccus sp. WB1 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 48000 | - |
x * 48000, SDS-PAGE | Paracoccus sp. WB1 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.3 | creatine + H2O | Paracoccus sp. WB1 | - |
sarcosine + urea | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.3 | Paracoccus sp. WB1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.3.3 | native enzyme 10.17fold to homogeneity by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration | Paracoccus sp. WB1 |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | additional information | - |
measurement of creatinine in a coupled assay with creatininase and sarcosine oxidase, overview | Paracoccus sp. WB1 |
3.5.3.3 | 6.1 | - |
purified enzyme | Paracoccus sp. WB1 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.3 | creatine + H2O | - |
Paracoccus sp. WB1 | sarcosine + urea | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.3.3 | ? | x * 48000, SDS-PAGE | Paracoccus sp. WB1 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 37 | - |
- |
Paracoccus sp. WB1 |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 25 | 50 | - |
Paracoccus sp. WB1 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 25 | 50 | purified enzyme, stable | Paracoccus sp. WB1 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 7.5 | - |
- |
Paracoccus sp. WB1 |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 5.5 | 9.5 | - |
Paracoccus sp. WB1 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.3 | 5.5 | 9.5 | purified enzyme, stable | Paracoccus sp. WB1 |