EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.9 | 2,4-Dinitrophenylhydrazine | can partially substitute for phenylhydrazine, 67% of the activity with phenylhydrazine | Phaseolus vulgaris | |
3.5.3.9 | L-arginine | can partially substitute for phenylhydrazine, 19% of the activity with phenylhydrazine | Phaseolus vulgaris | |
3.5.3.9 | L-lysine | can partially substitute for phenylhydrazine, 29% of the activity with phenylhydrazine | Phaseolus vulgaris | |
3.5.3.9 | phenylhydrazine | completely dependent on phenylhydrazine, which acts as an activator at low concentrations and decreases the affinity of the enzyme for the substrate at higher concentrations, the activation is reduced by pyridoxamine-5'-phosphate and pyridoxal-5'-phosphate | Phaseolus vulgaris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.9 | 2-mercaptoethanol | 21% inhibition at 1 mM | Phaseolus vulgaris | |
3.5.3.9 | Borate | 8% inhibition at 10 mM | Phaseolus vulgaris | |
3.5.3.9 | EDTA | complete inhibition at 10 mM, 85% inhibition at 5 mM, the activity is partially restored only by Mn2+, activity inhibited by EDTA is not recovered after EDTA removal by dialysis | Phaseolus vulgaris | |
3.5.3.9 | hydroxylamine | 67% inhibition at 1 mM | Phaseolus vulgaris | |
3.5.3.9 | L-Asp | 29% inhibition at 10 mM | Phaseolus vulgaris | |
3.5.3.9 | NEM | 15% inhibition at 5 mM | Phaseolus vulgaris | |
3.5.3.9 | nitrate | inhibits the enzyme, overview | Phaseolus vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.3.9 | Mn2+ | required | Phaseolus vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.9 | allantoate + 2 H2O | Phaseolus vulgaris | allosteric regulation of the enzyme suggesting a critical role in the regulation of ureide degradation | ureidoglycolate + 2 NH3 + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.3.9 | Phaseolus vulgaris | - |
cv. Great Northern | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.3.9 | native enzyme 22.4fold by ultracentrifugation and batch separation using an anion exchanger resin | Phaseolus vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.3.9 | additional information | the activity is lower in extracts obtained from leaves and fruits of French bean grown with nitrate than in plants actively fixing nitrogen | Phaseolus vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.3.9 | 0.365 | - |
partially purified enzyme | Phaseolus vulgaris |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.3.9 | -20°C, partially purified enzyme, retains the activity for 11 days | Phaseolus vulgaris |
3.5.3.9 | 4°C, partially purified enzyme, retains the activity for 4 days | Phaseolus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.3.9 | allantoate + 2 H2O | - |
Phaseolus vulgaris | ureidoglycolate + 2 NH3 + CO2 | - |
? | |
3.5.3.9 | allantoate + 2 H2O | allosteric regulation of the enzyme suggesting a critical role in the regulation of ureide degradation | Phaseolus vulgaris | ureidoglycolate + 2 NH3 + CO2 | - |
? | |
3.5.3.9 | additional information | no activity with ureidoglycolate | Phaseolus vulgaris | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.3.9 | allantoate-degrading enzyme | - |
Phaseolus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.9 | 35 | - |
- |
Phaseolus vulgaris |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.3.9 | 65 | - |
30 min, inactivation | Phaseolus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.3.9 | 7 | - |
activity is maximal in the buffers triethanolamine, TES, HEPES, or phosphate, and 25% lower in MES buffer | Phaseolus vulgaris |