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Literature summary extracted from

  • Werner, A.K.; Sparkes, I.A.; Romeis, T.; Witte, C.P.
    Identification, biochemical characterization, and subcellular localization of allantoate amidohydrolases from Arabidopsis and soybean (2008), Plant Physiol., 146, 418-430.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.5 gene aln, transient expression in Nicotiana tabacum in the endoplasmic reticulum, occasionally also in the Golgi apparatus or the peroxisomes Arabidopsis thaliana
3.5.3.9 transient expression in Nicotiana tabacum as StrepII-tagged enzyme using the Agrobacterium tumefaciens transfection method Glycine max
3.5.3.9 transient expression in Nicotiana tabacum as StrepII-tagged enzyme using the Agrobacterium tumefaciens transfection method, co-expression of YFP-tagged enzyme in Nicotiana tabacum plants with endoplasmic reticulum and peroxisomal markers Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.5 additional information a T-DNA insertion allantoinase mutant is unable to grow on 10 mM allantoin as the sole nitrogen source, although it still shows some root elongation with a few plants reaching the four-leaf stage, the mutant is rescued by a C-terminally tagged Arabidopsis thaliana AtAln variant, overview Arabidopsis thaliana
3.5.3.9 additional information an N-terminally truncated version of AAH-YFP lacking the first 71 amino acids exclusively localized to the cytosol Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.9 Borate
-
 Arabidopsis thaliana
3.5.3.9 Borate
-
 Glycine max
3.5.3.9 fluoride
-
 Arabidopsis thaliana
3.5.3.9 fluoride
-
 Glycine max
3.5.3.9 L-Asn inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition Arabidopsis thaliana
3.5.3.9 L-Asn inhibition via chelating Mn2+, allantoate protects and reverses, competitive or mixed-type inhibition Glycine max
3.5.3.9 L-Asp inhibition via chelating Mn2+, allantoate protects and reverses Arabidopsis thaliana
3.5.3.9 L-Asp inhibition via chelating Mn2+, allantoate protects and reverses Glycine max
3.5.3.9 additional information no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate Arabidopsis thaliana
3.5.3.9 additional information no inhibition by beta-mercaptoethanol, allantoin, urea, N-alpha-acetyl-L-Asn and N-alpha-acetyl-D-Asn, nitrate, and by phenylphosphorodiamidate Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.2.5 endoplasmic reticulum
-
 Glycine max 5783
-
3.5.2.5 endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-
3.5.3.9 endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-
3.5.3.9 endoplasmic reticulum
-
 Glycine max 5783
-
3.5.3.9 Golgi apparatus
-
 Arabidopsis thaliana 5794
-
3.5.3.9 Golgi apparatus
-
 Glycine max 5794
-
3.5.3.9 peroxisome
-
 Arabidopsis thaliana 5777
-
3.5.3.9 peroxisome
-
 Glycine max 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.9 Mn2+
-
 Arabidopsis thaliana
3.5.3.9 Mn2+ required for activity Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.9 51900
-
 x * 51900, SDS-PAGE Glycine max
3.5.3.9 53100
-
 x * 53100, SDS-PAGE Arabidopsis thaliana
3.5.3.9 100000
-
 gel filtration Glycine max
3.5.3.9 103000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.5 allantoin + H2O Arabidopsis thaliana
-
 allantoate
-
 ?
3.5.3.9 allantoate + 2 H2O Arabidopsis thaliana allantoate degradation is required to recycle purine-ring nitrogen in plants (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 allantoate + 2 H2O Glycine max allantoate degradation is required to recycle purine-ring nitrogen in plants (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.5 Arabidopsis thaliana
-
-
-
3.5.2.5 Arabidopsis thaliana
-
 gene aln
-
3.5.2.5 Glycine max
-
-
-
3.5.3.9 Arabidopsis thaliana O49434 ecotype Columbia
-
3.5.3.9 Glycine max A9GYV1 cv. ‘Williams 82’, gene aah
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.9 recombinant StrepII-tagged enzyme from Nicotiana tabacum leaf extracts to homogeneity by streptavidin affinity chromatography Arabidopsis thaliana
3.5.3.9 recombinant StrepII-tagged enzyme from Nicotiana tabacum leaf extracts to homogeneity by streptavidin affinity chromatography Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.2.5 leaf
-
 Glycine max
-
3.5.2.5 leaf
-
 Arabidopsis thaliana
-
3.5.3.9 leaf
-
 Arabidopsis thaliana
-
3.5.3.9 leaf
-
 Glycine max
-
3.5.3.9 additional information distribution, overview Arabidopsis thaliana
-
3.5.3.9 additional information distribution, overview Glycine max
-
3.5.3.9 root
-
 Arabidopsis thaliana
-
3.5.3.9 root
-
 Glycine max
-
3.5.3.9 seedling
-
 Arabidopsis thaliana
-
3.5.3.9 seedling
-
 Glycine max
-
3.5.3.9 shoot
-
 Arabidopsis thaliana
-
3.5.3.9 shoot
-
 Glycine max
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.5 (S)-allantoin + H2O
-
 Glycine max allantoate
-
 ?
3.5.2.5 (S)-allantoin + H2O
-
 Arabidopsis thaliana allantoate
-
 ?
3.5.2.5 allantoin + H2O
-
 Arabidopsis thaliana allantoate
-
 ?
3.5.3.9 allantoate + 2 H2O allantoate degradation is required to recycle purine-ring nitrogen in plants Arabidopsis thaliana (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 allantoate + 2 H2O allantoate degradation is required to recycle purine-ring nitrogen in plants Glycine max (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 allantoate + 2 H2O an ureide Arabidopsis thaliana (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 allantoate + 2 H2O an ureide Glycine max (S)-ureidoglycolate + 2 NH3 + CO2
-
 ?
3.5.3.9 additional information substrate specificity, overview, no activity with N-alpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly Arabidopsis thaliana ?
-
 ?
3.5.3.9 additional information substrate specificity, overview, no activity with N-lpha-carbamoyl-L-Asp, N-alpha-carbamoyl-L-Ala, and N-alpha-carbamoyl-Gly Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.9 ? x * 53100, SDS-PAGE Arabidopsis thaliana
3.5.3.9 ? x * 51900, SDS-PAGE Glycine max

Synonyms

EC Number Synonyms Comment Organism
3.5.2.5 ALN
-
 Glycine max
3.5.2.5 ALN
-
 Arabidopsis thaliana
3.5.3.9 AAH
-
 Arabidopsis thaliana
3.5.3.9 AAH
-
 Glycine max
3.5.3.9 allantoate amidohydrolase
-
 Arabidopsis thaliana
3.5.3.9 allantoate amidohydrolase
-
 Glycine max

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.9 30
-
 assay at Arabidopsis thaliana
3.5.3.9 30
-
 assay at Glycine max

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.9 8
-
 assay at Arabidopsis thaliana
3.5.3.9 8
-
 assay at Glycine max

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.3.9 0.032
-
 pH 8.0, 30°C Arabidopsis thaliana fluoride
3.5.3.9 0.0425
-
 pH 8.0, 30°C Glycine max fluoride