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Literature summary extracted from

  • Brugiere, N.; Humbert, S.; Rizzo, N.; Bohn, J.; Habben, J.E.
    A member of the maize isopentenyl transferase gene family, Zea mays isopentenyl transferase 2 (ZmIPT2), encodes a cytokinin biosynthetic enzyme expressed during kernel development. Cytokinin biosynthesis in maize (2008), Plant Mol. Biol., 67, 215-229.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.27 construct consisting of the 35S promoter fused to the first intron of the maize alcohol dehydrogenase gene, fused to the isoform IPT2 coding sequence and a terminator. Ectopic expression in Arabidopsis thaliana Zea mays
2.5.1.112 expressed in Escherichia coli BL21-Star cells Zea mays

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.27 additional information ectopic expression of isoform IPT2 in calli and in planta of Arabidopsis thaliana creates phenotypes consistent with cytokinin overproduction Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.112 dimethylallyl diphosphate + ADP Zea mays
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + AMP Zea mays low efficiency diphosphate + N6-(dimethylallyl)adenosine phosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP Zea mays
-
 diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 additional information Zea mays 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Zea mays B2XWS4 isoform isopentenyl transferase 2, IPT2
-
2.5.1.112 Zea mays
-
 cultivar B73
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.112 Talon metal affinity column chromatography Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.27 embryo at 8-10 days after pollination the endosperm and especially the basal transfer cell layer is a major site of IPT2 expression, this expression persists in the basal transfer cell layer and the developing embryo into later kernel development stages Zea mays
-
2.5.1.27 kernel strong expression of isoform IPT2 in developing kernels. At 8-10 days after pollination the endosperm and especially the basal transfer cell layer is a major site of IPT2 expression, this expression persists in the basal transfer cell layer and the developing embryo into later kernel development stages Zea mays
-
2.5.1.27 leaf very low level of isoform IPT2 protein Zea mays
-
2.5.1.27 root very low level of isoform IPT2 protein Zea mays
-
2.5.1.27 stem very low level of isoform IPT2 protein Zea mays
-
2.5.1.112 embryo
-
 Zea mays
-
2.5.1.112 endosperm
-
 Zea mays
-
2.5.1.112 kernel strong expression in developing kernel Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + ADP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.27 dimethylallyl diphosphate + AMP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-phosphate
-
 ?
2.5.1.27 dimethylallyl diphosphate + ATP ADP and ATP are preferred over AMP Zea mays diphosphate + N-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.27 additional information no substrate: 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate Zea mays ?
-
 ?
2.5.1.112 dimethylallyl diphosphate + ADP
-
 Zea mays diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + AMP low efficiency Zea mays diphosphate + N6-(dimethylallyl)adenosine phosphate
-
 ?
2.5.1.112 dimethylallyl diphosphate + ATP
-
 Zea mays diphosphate + N6-(dimethylallyl)adenosine 5'-triphosphate
-
 ?
2.5.1.112 additional information 1-hydroxy-2-methyl-2-buten-4-yl 4-diphosphate is not a substrate Zea mays ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.112 IPT2
-
 Zea mays
2.5.1.112 isopentenyl transferase 2
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.112 ADP the enzyme preferentially uses ADP and ATP over AMP Zea mays
2.5.1.112 ATP the enzyme preferentially uses ADP and ATP over AMP Zea mays

Expression

EC Number Organism Comment Expression
2.5.1.112 Zea mays enzyme expression is developmentally regulated in kernel tissues and coincides with cytokinin accumulation. The expression gradually increases from 6 to 8 days after pollination (DAP), peaks at 8 DAP, and decreases gradually until 14 DAP before increasing again at later stages (15-34 DAP) up

General Information

EC Number General Information Comment Organism
2.5.1.112 physiological function the enzyme is expressed during kernel development Zea mays