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Literature summary extracted from

  • Levac, D.; Murata, J.; Kim, W.S.; De Luca, V.
    Application of carborundum abrasion for investigating the leaf epidermis: molecular cloning of Catharanthus roseus 16-hydroxytabersonine-16-O-methyltransferase (2008), Plant J., 53, 225-236.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.94 16-hydroxytabersonine-16-O-methyltransferase is isolated and cloned as a His-tagged fusion protein in Escherichia coli Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.94 S-adenosyl-L-homocysteine product inhibition Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.94 0.0026
-
16-hydroxytabersonine
-
Catharanthus roseus
2.1.1.94 0.0217
-
S-adenosyl-L-methionine
-
Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.94 40000
-
SDS-PAGE Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.94 Catharanthus roseus B0EXJ8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.94 using PD-10 column chromatography, Sephadex G150 column chromatography, adenosine-agarose affinity resin and Mono Q anion exchange column chromatography Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.94 leaf predominatly expressed in leaf epidermis Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.94 0.000417
-
16-hydroxytabersonine as a substrate Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.94 S-adenosyl-L-methionine + 16-hydroxytabersonine 16OMT is highly specific for its alkaloid substrate 16-hydroxytabersonine Catharanthus roseus S-adenosyl-L-homocysteine + 16-methoxytabersonine
-
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Synonyms

EC Number Synonyms Comment Organism
2.1.1.94 16-hydroxytabersonine-16-O-methyltransferase
-
Catharanthus roseus
2.1.1.94 16OMT
-
Catharanthus roseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.94 30
-
assay at Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.94 7.5
-
assay at Catharanthus roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.94 7 7.5
-
Catharanthus roseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.1.94 0.006
-
S-adenosyl-L-homocysteine product inhibition Catharanthus roseus