EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.94 | 16-hydroxytabersonine-16-O-methyltransferase is isolated and cloned as a His-tagged fusion protein in Escherichia coli | Catharanthus roseus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.94 | S-adenosyl-L-homocysteine | product inhibition | Catharanthus roseus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.94 | 0.0026 | - |
16-hydroxytabersonine | - |
Catharanthus roseus | |
2.1.1.94 | 0.0217 | - |
S-adenosyl-L-methionine | - |
Catharanthus roseus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.94 | 40000 | - |
SDS-PAGE | Catharanthus roseus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.94 | Catharanthus roseus | B0EXJ8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.1.1.94 | using PD-10 column chromatography, Sephadex G150 column chromatography, adenosine-agarose affinity resin and Mono Q anion exchange column chromatography | Catharanthus roseus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.1.1.94 | leaf | predominatly expressed in leaf epidermis | Catharanthus roseus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.1.1.94 | 0.000417 | - |
16-hydroxytabersonine as a substrate | Catharanthus roseus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.94 | S-adenosyl-L-methionine + 16-hydroxytabersonine | 16OMT is highly specific for its alkaloid substrate 16-hydroxytabersonine | Catharanthus roseus | S-adenosyl-L-homocysteine + 16-methoxytabersonine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.1.1.94 | 16-hydroxytabersonine-16-O-methyltransferase | - |
Catharanthus roseus |
2.1.1.94 | 16OMT | - |
Catharanthus roseus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.1.1.94 | 30 | - |
assay at | Catharanthus roseus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.94 | 7.5 | - |
assay at | Catharanthus roseus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.1.1.94 | 7 | 7.5 | - |
Catharanthus roseus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.1.1.94 | 0.006 | - |
S-adenosyl-L-homocysteine | product inhibition | Catharanthus roseus |