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Literature summary extracted from
- The role of 5-adenylylsulfate reductase in the sulfur assimilation pathway of soybean: molecular cloning, kinetic characterization, and gene expression (2008), Phytochemistry, 69, 356-364.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.4.9 | expression in Escherichia coli | Glycine max |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.4.9 | AMP | competitive inhibitor with respect to 5'-adenylyl sulfate | Glycine max |  |
| 1.8.4.9 | additional information | sulfite (up to 100 mM) has no inhibitory effect | Glycine max |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.9 | 0.0112 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: dithiothreitol | Glycine max | |
| 1.8.4.9 | 0.0174 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: glutathione | Glycine max | |
| 1.8.4.9 | 0.495 | - |
glutathione | 30°C, pH 8.5 | Glycine max | |
| 1.8.4.9 | 0.595 | - |
dithiothreitol | 30°C, pH 8.5 | Glycine max | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.9 | 46000 | - |
2 * 46000, SDS-PAGE | Glycine max |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.4.9 | Glycine max | - |
L. Merr., cv Williams 82 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.4.9 | - |
Glycine max |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.8.4.9 | root | cold-treatment increases expression and the total activity of APS reductase in root tissues | Glycine max | - |
| 1.8.4.9 | seed | sulfur and phosphorus deprivation increases expression level, while nitrogen starvation represses APS reductase mRNA transcript and protein levels | Glycine max | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.4.9 | 0.207 | - |
- |
Glycine max |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.4.9 | 5'-adenylyl sulfate + glutathione | - |
Glycine max | AMP + sulfite + glutathione disulfide | - |
? | |
| 1.8.4.9 | 5'-adenylylsulfate + dithiothreitol | - |
Glycine max | adenosine 5'-monophosphate + sulfite + oxidized dithiothreitol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.4.9 | dimer | 2 * 46000, SDS-PAGE | Glycine max |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.4.9 | 5'-adenylylsulfate reductase | - |
Glycine max |
| 1.8.4.9 | APS reductase | - |
Glycine max |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.9 | 8.4 | - |
glutathione | 30°C, pH 8.5 | Glycine max | |
| 1.8.4.9 | 8.4 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: dithiothreitol | Glycine max | |
| 1.8.4.9 | 10.1 | - |
dithiothreitol | 30°C, pH 8.5 | Glycine max | |
| 1.8.4.9 | 11.3 | - |
5'-adenylyl sulfate | 30°C, pH 8.5, cosubstrate: glutathione | Glycine max | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.4.9 | 1 | - |
AMP | - |
Glycine max | |
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